26-nor-8-羟基-α-芒柄花醇对体外培养成骨细胞活性影响的研究  

Effects of 26-nor-8-hydroxy-α-onocerin on Osteoblast Activity in vitro

作　　者：魏甜甜[1] 李俊[1] 宋超[1] 唐俊龙[1] 易继凌[1] 杨光中[2] 王朝元[1] 

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]中南民族大学药学院

出　　处：《中国中医骨伤科杂志》2012年第4期4-7,共4页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：湖北省自然科学基金(合同号:2009CDZ033)

摘　　要：目的:研究26-nor-8-羟基-α-芒柄花醇(26-nor-8-hydroxy-α-onocerin,26HO)对成骨细胞活性的影响。方法:采用MTT法研究不同浓度的26HO对成骨细胞增殖率的影响;并用Real-Time PCR研究不同浓度的26HO对成骨细胞成骨相关基因表达量的影响。结果:20μM/L和40μM/L的26HO短期处理(1d)能够促进成骨细胞增殖,长期处理对成骨细胞增殖活性没有显著影响;26HO对骨相关基因(ALP、OCN、Col-I)表达的影响与成骨细胞的成熟状态有关。而对AP-1转录因子家族基因中Fos亚家族成员(c-fos、Fra-1、Fra-2)等基因mRNA表达的影响与26HO浓度有关,与处理时间无关;而对AP-1转录因子家族基因中Jun亚家族成员(c-Jun和Jun-D)基因mRNA表达的影响与26HO浓度无关,与处理时间有关。结论:26HO具有成骨活性。Objective：To investigate the effects of 26-nor-8-hydroxy-α-onocerin（26HO） on osteoblast activity.Methods：MTT assay was used to analyze the effect of 26HO on osteoblast proliferation and Real-Time PCR was applied to explore the effect of 26HO on the expression of osteogenic genes in osteoblasts.Results：One-day treatment of 20μM/Land 40μM/L 26HO significantly promoted osteoblast proliferation,but long-term intervention showed no obvious effect on osteoblast proliferation.The effect of 26HO on bone-related gene expression was related to the maturation status of osteoblast being treated.The effect of 26HO on the expression of fos sub-family of AP-1 transcription factor family is related to concentration of 26HO but not treatment period,while the effect of 26HO on the expression of Jun sub-family of AP-1 transcription factor family was related to treatment period but not concentration of 26HO.Conclusion：26HO has osteogenic activity.

关 键 词：26-nor-8-羟基-α-芒柄花醇 成骨细胞 细胞增殖 成骨活性基因表达 

分 类 号：Q813.1[生物学—生物工程]