免疫印迹技术在老年人梅毒螺旋体抗体阳性标本中的临床应用  

Clinical application of Western blot technology in old age group with Treponema pallidum antibody positive sampies

作　　者：韩晓峰[1] 刘孙琴[1] 

机构地区：[1]武汉科技大学附属天佑医院检验科,湖北武汉430064

出　　处：《医学检验与临床》2012年第1期29-30,41,共3页Medical Laboratory Science and Clinics

摘　　要：目的探讨免疫印迹技术在老年人梅毒螺旋体抗体阳性标本中的临床应用，减少假阳性，避免误诊。方法将ELISA法检测出的老年人梅毒螺旋体抗体阳性标本78例，用免疫印迹法（WB）和TPPA法进行确认实验。结果ELISA法检出阳性78例，WB法确认阳性68例，假阳性率12．82％；TPPA确认阳性69例，假阳性率11．53％；ELISA法假阳性的SCO值与真阳性的SCO值差别无统计学意义（P〉0．05），WB法与TPPA法确认的阳性率差别无统计学意义（P〉0．05）。结论为提高老年人梅毒检测的准确性，对ELISA法检测的阳性标本，结合临床表现与病史，对有疑问的标本，用WB法进行确认，排除假阳性。Objective To Study clinical Application of Western blot for the old age group with Treponema pallidum antibody positive sample, reduce the false positive to avoid misdiagnosis. Methods The 78 c_ases positive samples of the old age group Treponema pallidum antibody were detected by the enzyme - linked immunosorbent assay （ELISA）, the positive samples were confirmed by WB and TPPA. Result The 78 cases positive samples were detected by ELISA, the 68 cases were confirmed by WB method, falsepositive rate was 12.82% ; the 69 cases were confirmed by TPPA method, false - positive rate was 11.53%, The SCO value of a false positive and true positive when were detected by ELISA was not statistically significant,the difference of positive rate between WB method and TPPA method was not statistically significant （x^2 = 0.06, P 〉 0.05 ）. Conclusion To improve the accuracy of syphilis testing the elderly, the positive result by ELISA testing must be carefully combined with the clinical diagnosis and disease history, when result have question, using W-B method confirm positive samples, to eliminate false positive.

关 键 词：梅毒螺旋体(TP) 酶联免疫吸附试验(ELISA) 梅毒螺旋体颗粒凝聚试验(TPPA)免疫印迹 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]