化橘红GAP基地土壤绿僵菌的分离鉴定  

Isolation and identification of Metarhizium from Citrus grandis ‘tomentosa’ GAP base

作　　者：马维思[1] 徐江[1] 乔海莉[1] 陈君[1] 李向明[2] 覃蓉敏[2] 程惠珍[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室,北京100193 [2]化州市绿色生命有限公司,广东化州525100

出　　处：《中国中药杂志》2012年第7期887-891,共5页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目(2006BAI09B04);高校博士学科点专项科研基金项目(20101106110040)

摘　　要：目的:从化橘红GAP基地土壤中分离、鉴定绿僵菌,为进一步利用绿僵菌防治化橘红害虫奠定基础。方法:采用黄粉虫诱集法从40份土壤样品中诱集、分离绿僵菌,通过形态特征和ITS序列比对鉴定其种类。结果:从化橘红GAP基地土壤中共分离出18个绿僵菌菌株,经鉴定均为金龟子绿僵菌小孢变种Metarhizium anisopliae var.anisoplia。结论:化橘红GAP基地环境适于金龟子绿僵菌小孢变种生存,土壤中拥有丰富的绿僵菌资源,这为进一步利用绿僵菌防治化橘红害虫奠定基础。A type of entomopathogenic fungus of soil in Citrus grandis ‘tomentosa’ production base was isolated and identified with morphological and molecular biological methods,including pathogenesis,spore characteristic and ITS sequence analysis were conducted.The results showed that eighteen entomopathogenic fungi strains were isolated from the Tenebrio molitor infected in the soil samples,which were identified as Metarhizium anisopliae var.anisopliae.Based on results above,we concluded that there was quantity of Metarhizium resources in this area.These provided the useful information for controlling some pests of C.grandis by using these strains of fungus.

关 键 词：化橘红 绿僵菌 分离 鉴定 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]