RSLC快速测定多穗柯甜茶中5种甜味成分  

Quick determination of five sweet constituents in Duosuike Tiancha by RSLC

作　　者：何春年[1,2] 彭勇[1,2] 肖伟[1,2] 胡玉丽[3] 肖培根[1,2] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]国家教育部中草药物质基础与资源利用重点实验室,北京100193 [3]包头医学院,内蒙古包头014060

出　　处：《中国中药杂志》2012年第7期961-965,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30530860)

摘　　要：多穗柯甜茶中含有多种二氢查尔酮类甜味成分,也是其主要活性成分,为了全面评价多穗柯甜茶的质量,该文选择根皮苷、三叶苷、3'-O-乙酰基根皮苷、2'-O-乙酰基根皮苷、根皮素5种甜味成分为指标进行测定。采用超快速液相色谱法(RSLC),Waters Acquity UPLCBEH C18色谱柱(2.1 mm×100 mm,1.7μm),乙腈-水(25∶75)等度洗脱,流速0.5 mL.min-1,检测波长285 nm,柱温40℃。结果表明5种甜味成分的线性关系良好(>0.9991),线性范围(g.L-1)和加样回收率分别为0.022 2～0.444(98.37%),0.102 8～4.112(97.32%),0.003 39～0.067 68(96.77%),0.005 1～0.204(98.85%)和0.000 538～0.010 76(100.91%)。多穗柯甜茶中这5种成分的质量分数范围(mg.g-1)分别为7.83～62.37,114.24～272.35,0～1.02,0～5.11,0.10～1.19,并且它们的含量变化与样品规格、采收时间呈一定的规律性。该实验方法的灵敏度和准确度均较好,采用等度洗脱能够在6 min内完成1份样品的快速测定,适合多穗柯甜茶中5种成分的快速分析。Duosuike Tiancha contain multiple dihydrochalcone sweet constituents,which are mainly active constituents.For the purpose of overall assessment on quality Duosuike Tiancha,5 sweet dihydrochalcones in Duosuike Tiancha,phloridzin,phloretin-4′-β-D-glucopyranoside,3′-O-acetylphloridzin,2′-O-acetylphloridzin and phloretin are determined as indicators.The separation was carried out through a isocratic elution using a Waters Acquity UPLCBRH C18（2.1 mm×100 mm,1.7 μm） column and a mobile phase consisting of water（75%） and acetonitrile（25%） at a flow rate of 0.5 mL·min-1.The detection wavelength was set at 285 nm.The column temperature was 40 ℃.Under the optimized conditions,all the 5 sweet constituents were successfully separated with in 6 min,and good linearity（r20.999 1） was achieved.The linear range（g·L-1） and recoveries were tested with results of 0.022 2-0.444（98.37%）,0.102 8-4.112（97.32%）,0.003 39-0.067 68（96.77%）,0.005 1-0.204（98.85%） and 0.000 538-0.010 76（100.91%） respectively.The results indicate that the content of the 5 dihydrochalcones were 7.83-62.37,114.24-272.35,0-1.02,0-5.11 and 0.10-1.19 mg·g-1,respectively.Furthermore,with certain regularity between their content and the sample size,harvest time.The separation and analysis method are fast and simple,as evidenced by the fact that the gradient elution is adopted to rapidly determine one sample within six minutes.Therefore,it can be used for dsetermine 5 sweet dihydrochalcones Duosuike Tiancha.

关 键 词：多穗柯甜茶 木姜叶柯 超快速液相 根皮苷 三叶苷 根皮素 

分 类 号：R284[医药卫生—中药学]