戊己丸不同配伍对小檗碱、巴马汀吸收的影响  

Impact on absorption of berberine and palmatine in different compatibilities of Wuji pill

作　　者：王怡薇[1] 李玉洁[1] 王彦礼[1] 杨伟鹏[1] 陈颖[1] 张东[1] 杨庆[1] 张英丰[2] 李涛[1] 朱晓新[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]广州中医药大学,广东广州510006

出　　处：《中国中药杂志》2012年第7期985-990,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金重点项目(30930114);国家"重大新药创制"科技重大专项(2009ZX09301-005-009);中国中医科学院基本科研业务费自主选题项目(ZZ2006114;ZZ20090211)

摘　　要：目的:应用大鼠原位循环肠肝灌流模型,考察戊己丸不同配伍对戊己丸中黄连代表成分小檗碱、巴马汀的吸收的影响。方法:采用L9(34)正交设计得到戊己丸12个配伍方(3个单味药和9个配伍方),经十二指肠给药,于不同时间(0,1,5,10,15,30,60,90,120 min)采集灌流液,LC-MS检测小檗碱、巴马汀的含量,计算其吸收分数,吸收速率常数(Ka)。结果:吴茱萸与小檗碱、巴马汀的吸收分数,吸收速率常数(Ka)之间呈负相关,并得到了促进小檗碱、巴马汀吸收的最优配伍比例黄连-吴茱萸-白芍3∶1∶3。结论:吴茱萸抑制黄连代表成分的吸收,而且黄连、吴茱萸、白芍配伍比例不同,小檗碱、巴马汀的吸收量、吸收速度也有较大差异。Objective： To observe the impact on absorption of berberine and palmatine in different compatibilities of Wuji pill by the perfused rat intestine-liver preparation.Method： Use L9（34） orthogonal design table,establish the perfused rat intestine-liver preparation,the twelve Wuji pill compatibilities duodenal administrated,collect the perfusate at different times points for LC-MS detection,calculate the absorbed score,Ka.Result： Evodiae Fructus and the absorption score,Ka of berberine and palmatine are inverse correlated.The most superior portion which promote the absorption is Coptidis Rhizoma-Evodiae Fructus-Paeoniae Radix Alba 3∶1∶3.Conclusion： Evodiae Fructus suppressed the absorption of berberine and palmatine.With the different portion the absorption also have big different.

关 键 词：肠肝灌流 戊己丸 盐酸小檗碱 盐酸巴马汀 吸收分数 吸收速率常数 

分 类 号：R285.5[医药卫生—中药学]