万胜化风丹对大鼠亚急性毒性的研究  

Studies on subacute toxicity of Wansheng Huafeng Dan in rats

作　　者：彭芳[1] 杨虹[2] 吴芹[3] 刘杰[3] 时京珍[2] 

机构地区：[1]贵阳中医学院生理教研室,贵州贵阳550002 [2]贵阳中医学院实验中心基础医学实验室,贵州贵阳550002 [3]贵州省基础药理重点实验室,贵州遵义563003

出　　处：《中国中药杂志》2012年第7期1017-1022,共6页China Journal of Chinese Materia Medica

基　　金：贵州省国际科技合作项目(黔科合外G字[2011]7020);贵州省中药现代化项目(2010-5)

摘　　要：目的:比较朱砂及万胜化风丹与氯化汞、甲基汞的亚急性毒性作用。方法:健康SD大鼠连续每天分别灌胃万胜化风丹(0.42 g.kg-1)、朱砂(0.15 g.kg-1)、硫化汞(0.15 g.kg-1)、氯化汞(0.02 g.kg-1)、甲基汞(0.001 g.kg-1),以及生理盐水21 d,观察大鼠一般情况,监测体重,于末次给药后断头取血、肝脏、肾脏和脑组织,计算肝、肾指数,检测血清谷丙转氨酶、尿素氮、肌酐含量,检测肝、肾、脑组织中汞蓄积量,RT-PCR法检测肝脏金属硫蛋白-1(MT-1)、细胞色素P450基因亚型(Cyp1a1,Cyp2b1,Cyp2e1,Cyp3a2,Cyp4a10)mRNA的相对表达量。结果:氯化汞引起大鼠体重明显低于对照组,肝、肾汞蓄积量明显增多;甲基汞显著地引起大鼠肝、肾、脑组织中汞蓄积量增多;而万胜化风丹和朱砂组没有明显变化。然而,各组血液生化和组织病理学没有表现出显著差异。氯化汞和甲基汞均明显诱导大鼠肝脏MT-1 mRNA的表达。万胜化风丹、朱砂引起肝Cyp3a2 mRNA表达增加,而氯化汞和甲基汞抑制了Cyp2e1 mRNA的表达。结论:万胜化风丹以临床等效剂量灌胃大鼠3周,对大鼠的亚急性毒性作用远低于氯化汞、甲基汞。Objective： To compare sub-acute toxic effects of cinnabar and Wansheng Huafeng Dan with mercury chloride and methyl-mercury.Method： Healthy SD rats were orally administered with Wansheng Huafeng Dan（0.42 g·kg-1）,cinnabar（0.15 g·kg-1）,HgS（0.15 g·kg-1）,HgCl2（0.02 g·kg-1）,MeHg（0.001 g·kg-1） and saline for 21 days under observed and their weights were monitored.After the final administration,they were decapitated and their blood,liver,kidney and brain tissues were collected for calculating hepatic and renal indexes and detecting the contents of serum glutamic pyruvic transaminase,urea nitrogen and creatinine and the mercury accumulation in liver,kidney and brain tissues.Besides,relative expressions of liver metallothionein-1（MT-1） and cytochrome P450 gene subtypes（Cyp1a1,Cyp2b1,Cyp2e1,Cyp3a2,Cyp4a10） mRNA.Result： HgCl2 caused obvious weight lose in rats.Mercury contents in liver and kidney were markedly increased by HgCl2 and MeHg,and MeHg markedly increased mercury contents of brain either,but these advent effects were not notable in Wansheng Huafeng Dan and cinnabar groups.However,blood biochemistry and histopathology did not show significant changes in all groups.The expression of rat hepatic MT-1 mRNA was remarkably induced by both HgCl2 and MeHg.The expression of hepatic Cyp3a2 was increased by Wansheng Huafeng Dan and cinnabar,while the expression of Cyp2e1 was inhibited by HgCl2 and MeHg.Conclusion： The administration of Wansheng Huafeng Dan with equivalent dose for three weeks shows a much low sub-acute toxicity than HgCl2 and MeHg in rats.

关 键 词：万胜化风丹 朱砂 氯化汞 甲基汞 金属硫蛋白 亚急性毒性 

分 类 号：R114[医药卫生—卫生毒理学]