缺血再灌注损伤时肠道分泌型免疫球蛋白A及其分泌细胞的变化  

The alteration in intestinal secretory immunoglobulin A and its secreting cells during ischemia/reperfusion injury

作　　者：孙立群[1] 李维勤[2] 李秋荣[2] 李宁[2] 黎介寿[2] 

机构地区：[1]南京医科大学第二附属医院ICU,南京210011 [2]南京军区南京总医院全军普通外科研究所,南京210002

出　　处：《解放军医学杂志》2012年第4期293-296,共4页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金重点项目(30830098);全军"十一五"医药卫生科研基金(06G041)~~

摘　　要：目的观察缺血再灌注(I/R)时肠道分泌型免疫球蛋白A(sIgA)及其分泌细胞的变化。方法将48只BALB/c小鼠随机分为对照组与再灌注2、6、12、24、72h组(n=8),检测远隔脏器(肝、脾、肠系膜淋巴结)细菌移位情况,ELISA法检测肠道sIgA水平变化,流式细胞仪检测肠相关淋巴组织中B细胞亚群改变。结果 I/R时发生细菌移位,肠道sIgA水平下降,二者呈明显负相关(r2=0.729)。随着I/R损伤的加重,肠相关淋巴组织中IgM+B220+细胞比例逐渐增高,IgA+B220+细胞比例逐渐降低,且在R12h组最为明显(P<0.01)。结论 I/R时肠相关淋巴组织中IgM+B细胞比例增高,IgA+B细胞比例下降,导致肠道sIgA水平下降,发生远隔脏器细菌移位。Objective To investigate the change in intestinal secretion immunoglobulin A（sIgA） level and IgA-secreting cells during ischemia/reperfusion（I/R） injury.Methods Forty-eight BALB/c mice were randomly divided into 6 experimental groups in accordance with different reperfusion times（R2h,R6h,R12h,R24h,and R72h group）,and one sham group（n=8）.Bacterial translocation to distant organs（lung,spleen,and mesenteric lymph nodes） was observed.The sIgA level of the intestinal tract was measured by enzyme-linked immunosorbent assay（ELISA）.The B cell subgroup in the lymphocytes related to the intestinal tract was measured by flow cytometry.Results The bacterial translocation occurred during I/R injury,and the intestinal sIgA level decreased,and they showed an obvious negative correlation（r2=0.729）.With the increase in intestinal I/R injury,the ratio of IgM＋B220＋ cells in the gut-associated lymphoid tissue increased,whereas the proportion of IgA＋B220＋ cells decreased.The most significant change was found in R12h group（P0.01）.Conclusions The proportion of IgM＋ B cells in the gut-associated lymphoid tissue increased,whereas that of IgA＋ B cells reduced during I/R injury.These phenomena may cause sIgA level to reduce and bacterial translocation of the distant organs to occur.

关 键 词：免疫球蛋白A 分泌 IgA+B细胞 IgM+B细胞 再灌注损伤 细菌移位 

分 类 号：R649.9[医药卫生—外科学]