胆道闭锁血清及肝脏中白介素-33的表达研究  

The expression of interleukin-33 in the serum and liver tissue of the patients with biliary atresia

作　　者：董瑞[1] 董岿然[1] 沈淳[1] 李凯[1] 郑珊[1] 肖现民[1] 赵瑞[1] 宋再[1] 孙松[1] 

机构地区：[1]复旦大学附属儿科医院外科 卫生部新生儿疾病重点实验室,上海201102

出　　处：《中华小儿外科杂志》2012年第4期259-262,共4页Chinese Journal of Pediatric Surgery

基　　金：基金项目：上海市科委重点项目（编号：09JC1402800）;国家自然科学基金项目（编号：30973139）

摘　　要：目的研究胆道闭锁（BA）血清及肝脏组织中白介素-33（IL-33）的表达及意义。方法选取18例BA与12例无黄疸症状且肝功能正常的胆总管囊肿（CC）患儿血清和肝脏标本进行对比研究。应用酶联免疫吸附试验检测血清中IL-33的表达水平。荧光定量RT-PCR技术和免疫印迹技术分别检测肝脏组织中IL-33mRNA及蛋白表达水平。结果BA组血清中IL-33的表达（791．0±22．22）pg／ml明显高于CC组的（607．1±20．68）pg／ml（P〈0．0001）；并与γ谷氨酰转移酶呈正相关（r=0．629，P=0．005）。BA组肝组织中IL-33mRNA表达0．0420±0．0061明显高于CC组的0．0220±0．0027（P=0．0181）。BA组肝组织中IL-33蛋白表达0．4683±0．0188亦明显高于CC组的0．3433±0．0293（P=0．0137）。结论IL-33在BA组血清及肝组织中表达均明显升高，可能在BA炎症发生及发展过程中发挥重要作用，值得进一步研究。Objective To study the expression pattern of interleukin-33 （IL-33） in the serum and liver tissue of the patients with biliary atresia （BA）. Methods Eighteen infants with biliary atresia, including 12 boys and 6 girls were recruited in this study. Their age ranged from 1 to 4 months old. The controls consisted of 12 children with choledochal cyst （CC）, including 5 boys and 7 girls. The controls＇ age ranged from 3 months to 4 years old. The controls were anicteric and had normal liver function. The diagnosis of BA and CC were confirmed by operation and histological examination. The serum level of IL-33 was measured by enzyme-linked irnmunosorbent assay. The mRNA of IL-33 in liver tissue was detected using Real-time quantitative reverse transcriptase-polymerase chain reaction. The IL-33 protein in liver tissue was examined by western blotting. Results The IL-33 level in serum of BA patients was significantly higher than that of CC patients （791.0 ± 22.22 pg/ml vs. 607. 1 ± 20. 68 pg/ml, P〈0. 0001）, and was positively correlated with γ-glutamyl transferase （r： 0. 629, P= 0. 005）. Compared with CC patients, the mRNA expression of IL-33 in liver tissue was significantly higher in BA （0. 0420 ± 0. 0061 vs. 0. 0220 ± 0. 0027, P = 0. 0181）, and the expression of IL-33 protein was also increased in BA patients （0. 4683 ± 0. 0188 vs. 0. 3433 ± 0. 0293, P = 0. 0137）. Conclusions The expression of IL-33 in the serum and liver tissue of the patients with BA is significantly elevated. This observation suggests IL-33 may play a important role in the pathogenesis of BA.

关 键 词：胆道闭锁 胆总管囊肿 白细胞介素类 Γ谷氨酰转移酶 

分 类 号：R726.5[医药卫生—儿科]