高粱靶斑病菌侵染对高粱光系统Ⅱ的影响  

Effects of Infection of Bipolaris Sorghicola on PSⅡ of Sorghum

作　　者：张园园[1] 徐秀德[2] 王振东[3] 张立军[3] 王丽娟[2] 徐婧[2] 刘可杰[2] 

机构地区：[1]沈阳农业大学农学院,辽宁沈阳110161 [2]辽宁省农业科学院创新中心,辽宁沈阳110161 [3]沈阳农业大学生物科学技术学院,辽宁沈阳110866

出　　处：《作物杂志》2012年第2期42-46,共5页Crops

基　　金：现代农业产业技术体系建设专项基金(CARS-06-02-01)

摘　　要：以高粱品种Tx622B为试验材料,通过测定叶片叶绿素含量、叶绿素荧光快速诱导动力学曲线、过氧化氢(H2O2)含量和丙二醛(MDA)含量,来研究高粱靶斑病菌[Bipolaris sorghicola(Lefebvre&Sherwin)Alcorn]侵染高粱叶片后对光系统II(PSII)结构和功能的影响。结果表明,受病原菌侵染的高粱叶片,H2O2和MDA含量均升高,膜脂过氧化的程度加剧;快速叶绿素荧光诱导动力学曲线发生明显变化,进一步的JIP测试分析表明病原菌侵染严重伤害了PSII供体侧,阻断了受体侧QA到QB的电子传递,降低了反应中心的活性。Using sorghum variety Tx622B as material, the effects of the infection of Bipolaris sorghicola on photosystem Ⅱ（PSⅡ） structure and function of sorghum leaves were studied by determining the chlorophyll content, chlorophyll fluorescence transient, hydrogen peroxide （ H2 O2 ） content and malondialdehyde （ MDA ） content in leaves of sorghum. The results showed that the leaves of Sorghum infected by B. sorghicola induced increasing in MDA content of sorghum leaves along with enhanced lipid peroxidation and chlorophyll fluorescence transient in leaves of sorghum. Furthermore, JIP-test analysis showed that the pathogen infection seriously hurt the donor side of PSII, blocked the QA to Qs electron transfer, reduced the activity of reaction center.

关 键 词：高粱靶斑病菌 光系统Ⅱ 快速叶绿 素荧光诱导 JIP测试 过氧化氢 

分 类 号：S435.14[农业科学—农业昆虫与害虫防治]