农杆菌介导大豆胚尖转化体系的研究  

Optimization of Agrobacterium-mediated Transformation System of Soybean Embryonic Tip

作　　者：郑丽红[1,2] 季静[2] 王罡[2] 关春峰[2] 王伟[2] 王萍[3] 

机构地区：[1]天津大学化工学院,天津300072 [2]天津大学遗传工程研究所,天津300072 [3]淮海工学院海洋学院,江苏连云港222005

出　　处：《作物杂志》2012年第2期76-79,共4页Crops

基　　金：转基因生物新品种培育重大专项(2008ZX08004-001);国家转基因生物新品种培育重大专项(2009ZX08010-013B)

摘　　要：以大豆"黑农37"胚尖为外植体,利用农杆菌介导法将抗除草剂基因EPSPS转入大豆,并对转化各培养阶段6-苄氨基腺嘌呤(6-BA)浓度进行了优化。结果表明:在预培养和共培养培养基中加入较高浓度6-BA有利于抗性芽的诱导;1mg/L6-BA和0.2mg/L吲哚丁酸(IBA)配合使用有利于抗性芽的伸长且提高了抗性芽的再生率;将抗性芽添加在1mg/L IBA的培养基中培养7d后转入不加任何激素的培养基中生根快且移栽后成活率高。PCR结果初步证明将EPSPS基因转入到大豆中。The herbicide resistant gene（EPSPS）was transferred into soybean embryonic tip using the Agrobacteriurn-mediated transformation, and the transformation system was optimized. The results showed that higher 6-BA concentrations in the pre-culture and co-culture medium could induce resistant shoots. The results also indicated that lmg/L 6-BA and 0.2mg/L IBA could improve regeneration rate of the resistant shoots and elongate resistant shoots length. The shoots rooted quickly when cultured in medium with 1 mg/L IBA for 7 days and then transferred to the medium without any hormones. PCR results indicated that the EPSPS gene had been transferred into soybean.

关 键 词：大豆 胚尖 农杆菌介导 6-BA 转化 体系 

分 类 号：S565.1[农业科学—作物学]