表达犬瘟热病毒H基因重组新城疫病毒的构建及其免疫原性研究  

Construction of Newcastle disease virus-vectored canine distemper virus(CDV) vaccine and induction of CDV neutralizing antibody

作　　者：田美杰[1] 葛金英[1] 王喜军[1] 冯娜[2] 马良[3] 帅磊[1] 苏华[1] 高玉伟[2] 夏咸柱[2] 步志高[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业部兽医公共卫生重点开放实验室,黑龙江哈尔滨150001 [2]军事医学科学院军事兽医研究所,吉林长春130062 [3]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国预防兽医学报》2012年第4期257-261,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金青年科学基金项目(30800816)

摘　　要：为研制安全、有效的新型犬瘟热疫苗,本研究利用新城疫病毒(NDV)LaSota弱毒疫苗株反向遗传操作系统,构建出表达犬瘟热病毒(CDV)弱毒疫苗株Rockborn-20/8血凝素(H)蛋白的重组病毒rLa-CDV-H,并对其生物学特性进行鉴定,评估其作为犬瘟热活载体疫苗的安全性和有效性。通过免疫荧光和western blot试验证明了CDV H蛋白的正确表达;重组病毒株保持了LaSota亲本株的低致病性和高滴度鸡胚生长特性;重组病毒rLa-CDV-H接种12周龄比格犬后,可以诱导显著的CDV中和抗体反应。本研究表明重组病毒rLa-CDV-H具有作为犬瘟热重组病毒活载体疫苗的潜力。In order to develop a safe and efficient canine distemper vaccine based on Newcastle disease virus（NDV） La Sota vaccine strain,we constructed and rescued a recombinant NDV which expressed hemagglutinin（H） protein of canine distemper virus（CDV,Rockborn-20/8 strain）,named rLa-CDV-H,by reverse genetics techniques.The expression of CDV H protein in recombinant virus was confirmed by western blot and indirect immunofluorescence assay.rLa-CDV-H retained its high-titer growth property and low pathogenicity in embryonated chicken eggs.Moreover,rLa-CDV-H induced significant CDV neutralizing antibodies in 12 week old dogs post-inoculation.This result indicated that rLa-CDV-H was able to induce efficiently immune responses in dogs,which showed that rLa-CDV-H had potential of being a novel CDV vaccine.

关 键 词：犬瘟热病毒 血凝素蛋白 重组新城疫病毒 

分 类 号：S852.65[农业科学—基础兽医学]