鸡黄病毒CJD05株E基因克隆与序列分析  

Cloning and sequence analysis of E gene of a chicken flavivirus isolate CJD05 in China

作　　者：王劭[1,2] 陈仕龙[1,2] 陈少莺[1,2] 林锋强[1,2] 程晓霞[1,2] 朱小丽[1] 江斌[1] 李兆龙[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350003 [2]福建省畜禽疫病防治工程技术研究中心,福建福州350013

出　　处：《中国预防兽医学报》2012年第4期317-319,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：福建省农科院创新团队项目(STIF-Y02);福建省公益类科研院所专项(2011R1025-2)

摘　　要：为了解福建省鸡黄病毒(CFV)CJD05株来源及其遗传进化关系,根据鸭黄病毒(DFV)BYD-1株E基因全序列设计合成1对引物,特异性扩增CFV CJD05株的E基因,并对其序列进行分析。结果表明克隆获得CFV CJD05株1 503 bp的E基因特异性目的条带,同源性分析表明CFV CJD05株E基因核苷酸序列与DFVBYD-1株、鹅黄病毒(GFV)JS804株的同源性分别为99.2%、99.3%,氨基酸的同源性分别为99.0%、98.6%,表明CFV CJD05株、DFV BYD-1株和GFV JS804株高度同源,与坦布苏病毒(TBSV)的同源性高于其它虫媒介黄病毒。To investigate the CJD05 chicken flavivirus（CFV） Fujian isolate and phylogenetic relationships,a pair of primers were designed and synthesized based on the sequences of the duck flavivirus（DFV） E gene,the E gene of CFV CJD05 was amplified by RT-PCR and cloned into pMDT-18 vector for sequencing.The results showed that the full length E gene was 1,503 bp,encoding a protein of 501 amino acids.Evolution analysis demonstrated that the E nucleotide sequence from isolate CJD05 shared 99.2% identity with DFV BYD-1 strain,99.3% with goose flavivirus（GFV） JS804 strain,and 99.0% and 98.6% amino acid sequence identities with DFV BYD-1 and GFV JS804 strain,respectively.Avian flavivirus is unusual in that it affects terrestrial bird and aquatic bird,the homology of E gene among avian flavivirus and Tembusu virus is higher than that among avian flavivirus and in other arthropod-borne flavivirus.

关 键 词：鸡黄病毒 E基因 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]