姜黄素对甲醛致A549细胞氧化损伤拮抗作用  

Antagonitic effect of curcumin on formaldehyde-induced oxidative damage in A549 cell

作　　者：陈鑫[1] 江中发[2] 李宁[2] 石玉琴[2] 隋妍[1] 张本延[1] 

机构地区：[1]武汉科技大学医学院预防医学系,湖北武汉430065 [2]湖北省疾病预防控制中心

出　　处：《中国公共卫生》2012年第4期491-492,共2页Chinese Journal of Public Health

基　　金：国家科技支撑计划项目(2006BAI19B05-2)

摘　　要：目的探讨姜黄素对甲醛致细胞氧化损伤的拮抗效应。方法采用A549细胞株作为实验材料,实验设对照组、0.1 mmol/L甲醛染毒组,姜黄素组(0.1 mmol/L甲醛+2.5～20.0 mg/L姜黄素),检测A549细胞中一氧化氮合酶(NOS),丙二醛(MDA),超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)活性。结果甲醛染毒组A549细胞SOD、NOS和GSH-Px活性分别为(21.79±1.13)、(1.88±0.16)与(27.83±0.2)U/mgprot,与对照组比较,SOD、NOS和GSH-Px活性明显下降(P<0.05),MDA含量[(3.87±0.153.87)nmol/mgprot]明显升高(P<0.05)。与甲醛染毒组比较,各姜黄素组GSH-Px活性上升、MDA含量下降(P<0.05),与对照组比较,40 mg/L姜黄素组GSH-Px活性、MDA含量差异无统计学意义(P>0.05)。结论姜黄素可提高A549细胞抗氧化酶活性,并存在剂量效应关系。Objective To explore the antagonistic effect of curcumin on formaldehyde-induced oxidative damage in cells.Methods A549 cells were used as experiental material and divided into normal control group,formaldehyde exposure group（0.1 mmol/L）,and curcumin-antagonized formaldehyde group.Nitric oxide synthase（NOS）,malondialdehyde（MDA）,superoxide dismutase（SOD）,and glutathione peroxidase（GSH-Px） levels were detected.Results For formaldehyde exposure group,SOD,NOS,and GSH-Px activity in A549 cells were 21.79±1.13,1.88±0.16,and 27.83±0.2 U/mgprot,respectively,and significantly decreased（P〈0.05） compared to those of the control group.Compared with that of the control group,MDA increased significantly（P〈0.05）.Compared with the formaldehyde exposure group,GSH-Px activity increased（P〈0.05）,MDA content decreased in curcumine group（P〈0.05）.Compared with those of the control group,GSH-Px activity and MDA content showed no significant difference in 40 mg/L curcumine group（P〈0.05）.Conclusion Curcumin can enhance antioxidant enzyme activity in A549 cells in a dose-effect relationship.

关 键 词：甲醛 姜黄素 氧化损伤 拮抗作用 

分 类 号：R114[医药卫生—卫生毒理学]