FS在人肺腺癌细胞中表达及其生物学作用  

Expression and role of follistatin in human lung adenocarcinoma cells

作　　者：孙洋[1] 陈芳芳[2] 李继茹[1] 李晨光[1] 柳忠辉[1] 崔雪玲[1] 

机构地区：[1]吉林大学白求恩医学院免疫学系,长春130021 [2]吉林大学中日联谊医院

出　　处：《中国公共卫生》2012年第4期511-512,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(31101039);吉林省科技厅课题(20110754)

摘　　要：目的探讨卵泡抑素(FS)在人肺腺癌细胞中的表达及其作用。方法免疫细胞化学染色检测肺腺癌细胞FS表达情况,细胞计数试剂盒测定细胞增殖,流式细胞术检测细胞凋亡。结果免疫细胞化学染色显示人肺腺癌细胞表达FS;40、50 ng/mL激活素A组肺腺癌细胞增殖〔(吸光度值A分别为(0.49±0.07)及(0.42±0.08))明显低于对照组(0.62±0.09);如果预先用抗FS单克隆抗体中和内源FS后,10 ng/mL激活素A也能抑制肺腺癌细胞增殖(0.48±0.06),由其诱导的肺腺癌细胞凋亡率(24.8±6.7)%明显高于单独激活素A组细胞凋亡率(7.3±2.4)%。结论肺腺癌细胞表达的FS有利于肺腺癌细胞生存,阻断FS分泌或其生物学作用对治疗肺腺癌可能具有重要意义。Objective To investigate expression and role of follistation（FS） in human lung adenocarcinoma cells.Methods FS expression in lung adenocarcinoma cells was detected with immunocytochemical staining;cell proliferation was assayed with cell counting-hit-8 and cell apoptosis was examined with flow cytometry.Results Immunocytochemical staining showed that FS was expressed in lung adenocarcinoma cells.Cell proliferation assay revealed that in 40 ng/ml and 50 ng/ml activin A groups,the proliferation of lung adenocarcinoma cells was 0.49±0.07 and 0.42±0.08,which were lower than that in control group（0.62±0.09）.The results indicated that after the usage of anti-FS monoclonal antibody to neutralize endogenous FS,10 ng/ml activin A could inhibit the proliferation of lung adenocarcinoma cells（0.48±0.06）,and the induced cells apoptosis（24.8±6.7） was higher than that in alone activin A group（7.3±2.4）.Conclusion The results demonstrate that the expressed FS is beneficial to survival of lung adenocarcinoma cells and the blockade of FS expression and action may provide effective ways for treatment of lung adenocarcinoma.

关 键 词：卵泡抑素 激活素A 肺腺癌 凋亡 

分 类 号：Q255[生物学—细胞生物学]