牛支原体新基因(P18)的克隆表达及活性鉴定  

Cloning, expression and characterization of a novel gene (P18) from Mycoplasma bovis

作　　者：宋志强[1] 李媛[1] 孙文静[2] 刘洋[1] 邹晓辉[1] 陈维[2] 周玉梅[1] 辛九庆[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/动物细菌病研究室/国家牛传染性胸膜肺炎指定检测实验室,黑龙江哈尔滨150001 [2]东北农业大学动物医学院,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2012年第3期184-187,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31072131)

摘　　要：牛支原体(M.bovis)可以引起犊牛肺炎、关节炎、乳腺炎、角膜结膜炎等疾病,是一种牛的重要呼吸道病原体,目前M.bovis粘附宿主细胞的机制还不明确。研究表明,M.bovis表面存在的多种蛋白与致病菌在宿主体内的侵袭力与传播能力有关。我们通过分离表达多个M.bovis基因,最终获得了一个表达纤溶酶原结合蛋白的新基因,并命名为P18。该基因表达大小约为67ku的重组蛋白。通过western blot鉴定,重组表达的P18蛋白可以被M.bovis抗体识别。进一步的试验表明,P18蛋白还具有纤溶酶原结合活性,从而推测该基因表达的蛋白可能是M.bovis的一个粘附相关因子。Mycoplasma bovis is the causative agent of M. bovis-associated disease （MbAD）. While the mechanisms by which M. bovis adherent host cells is remain unclear, our recent study provided that M. boris express variable surface lipop proteins （Vsps） is facilitated it invasion and dissemination in the infected host and speculated that there are other adhesion-related factors in existence of M. bovis. We cloned and expressed a novel gene of M. bovis, designate P18, which express a profibrinolysin binding protein according to the domain sequence analysis binding test. The P18 gene express a 67 ku recombinant protein. The recombinant P18 protein were detected by SDS-PAGE and confirmed by western blot with positive serum. There fore, we conjectured that P18 protein is one of M. bovis adhesion-related factors.

关 键 词：牛支原体 亚克隆 表达 粘附相关因子 

分 类 号：S852.4[农业科学—基础兽医学]