禽传染性喉气管炎病毒gD蛋白单克隆抗体的制备  

Preparation of monoclonal antibodies against infectious laryngotracheitis virus gD protein

作　　者：胡顺磊[1] 赵妍[1] 崔红玉[1] 石星明[1] 王玫[1] 张晶[1] 薛美[1] 张晓敏[2] 闫帅[1] 孔聪聪[1] 李巧玲[1] 牛秀杰[1] 王云峰[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽传染病研究室,黑龙江哈尔滨150001 [2]云南农业大学,云南昆明650201

出　　处：《中国预防兽医学报》2012年第3期232-234,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：兽医生物技术国家重点实验室基本科研业务费(SKLVBP201022);中央级公益性科研院所基本科研业务费项目(ZGKJ201003);黑龙江省杰出青年科学基金项目(JC200812)

摘　　要：为制备抗鸡传染性喉气管炎病毒(ILTV)糖蛋白gD的单克隆抗体(MAb),本研究通过原核表达gD重组蛋白,纯化后免疫6周龄雌性BALB/c小鼠,经细胞融合筛选获得一株稳定分泌抗ILTV gD蛋白的杂交瘤细胞株,MAb亚型经鉴定为IgG1,轻链为κ链。Western blot结果显示,这株杂交瘤细胞分泌的MAb能够识别ILTV。ILTV gD蛋白的MAb的制备,为ILTV检测方法的建立奠定了基础。To prepare monoclonal antibody （MAb） against glycoprotein D of infectious laryngotracheitis virus （ILTV）, the SP2/0 cells were fused with spleen cells of BALB/c mice immunized with the purified recombinant gD protein, a hybridoma cell line stably secreting antibody was obtained by scanning with indirect ELISA coating with purified ILTV as coating antigen. The MAb was identified to be IgG1 subclass with K light chain. Western blot analysis showed that MAb reacted positively with ILTV. The MAb developed could serve as a tool for further study on ILTV and development of detection method for ILTV infection.

关 键 词：传染性喉气管炎病毒 gD蛋白 原核表达 单克隆抗体 

分 类 号：S852.65[农业科学—基础兽医学]