实时定量聚合酶链反应检测干燥综合征患者外周血单个核细胞TCLl和THRA基因表达的研究  

TCL1 and THRA gene expression levels of peripheral blood mononuclear cell in patients with Sjogrensyndrome

作　　者：程永静[1] 王正[3] 王芳[1] 张春媚[1] 王抒[2] 黎建[2] 黄慈波[1] 

机构地区：[1]卫生部北京医院风湿免疫科,100730 [2]卫生部老年医学研究所生化室,100730 [3]苏州大学神经科学研究所生化室,100730

出　　处：《中华风湿病学杂志》2012年第4期220-223,共4页Chinese Journal of Rheumatology

基　　金：国家自然科学基金（31140008）;北京医院院内基金（bj-2008-85）

摘　　要：目的研究干燥综合征（ss）患者及健康志愿者外周血单个核细胞（PBMC）THRA，TCLl基因的差异表达，为探讨疾病的发病机制、诊断及治疗提供新的依据。方法选取40例原发性干燥综合征（Dss）患者，40名同年龄健康志愿者为研究对象，Ficoll密度梯度离心法分离PBMC，Trizol-步法提取mRNA，反转录至cDNA，实时定量聚合酶链反应（PCR）法检测PBMC中THRA，TCLl等基因的表达。采用秩和检验和t检验进行统计分析。结果ss患者PBMC中TCLl的mRNA表达（2．5±4．7）明显高于健康对照，二者之间差异有统计学意义（Z=2．045，P〈0．05）；而THRA的表达水平分别为（1．2＋0．8）差异无统计学意义。淋巴结病变活动指数2-3分的SS患者TCLl表达水平（4．4±3．8）明显高于淋巴结病变活动指数1-2分的患者（1．1±1．1），二者之间差异有统计学意义（f=-2．319，P〈0．05），合并及未合并甲状腺疾病的ss患者THRA表达水平差异无统计学意义。结论ss患者TCLl基因的表达明显高于健康对照，且与SS淋巴结病变活动指数有关，为进一步验证为疾病的发病机制，评估疾病的活动性及诊治提供了新的研究思路。Objective To identify novel markers such as THRA or TCL1 in Sjogren＇s syndrome （SS） patients to discriminate them from healthy volunteers. Methods Experimental group （n =40） and healthy volunteer group （n=40） were recruited based on strict inclusion and exclusion criteria. Peripheral blood samples （9 ml） were collected and peripheral blood mononuclear cells were separated by Ficoll gradient centrifu gation. RNA was extracted using Trizol reagent, and the expression of THRA, TCL1 in PBMCs was detected by realtime PCR. And the data were processed with Wilcoxon test and t test （P〈0.05）. Results TCL1 and THRA expression level are higher in SS patients （2.5±4.7） than healthy volunteers （Z=-2.045, P〈0.05）. The TCL1 expression level （4.4±3.8） is higher in high lymphadenopathy activation index patients （2 to 3 grade） than that in low lymphadenopathy activation index patients （grade 0 to 1 ） （t=-2.319, P〈0.05）. Conclusion TCL1 expression level is higher in SS patients, and TCL1 expression level is related to the severity of lympha denopathy, which provide a new platform of the study for the pathogenesis, disease severity evaluation, even diagnosis and treatment of SS.

关 键 词：干燥综合征 基因 外周血单个核细胞 

分 类 号：R593.2[医药卫生—内科学]