辛伐他汀与福辛普利对慢性心力衰竭大鼠心肌结缔组织生长因子的影响  

Effect of simvastatin and fosinopril on myocardial connective tissue growth factor in rats with chronic heart failure

作　　者：李秀华[1] 康大伟[1] 王莉[1] 谢亚芹[1] 

机构地区：[1]承德医学院附属医院老年病科,067000

出　　处：《中华老年心脑血管病杂志》2012年第4期347-350,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的探讨辛伐他汀和福辛普利单用及联合应用对慢性心力衰竭大鼠左心室心肌结缔组织生长因子(connectivetissue growth factor,CTGF)的影响。方法利用肾上腹主动脉缩窄法制作慢性心力衰竭模型,雄性Wistar大鼠50只随机分为假手术组、模型组、辛伐他汀组、福辛普利组、联合用药组(辛伐他汀+福辛普利),每组10只。观察各组大鼠左心室重量指数(LVMI)、左心室舒张末压(LVEDP)、左心室压力最大上升及下降速率(±dp/dt_(max));SP法检测各组大鼠左心室心肌CTGF表达,Western blot检测各组大鼠左心室心肌CTGF蛋白表达水平,RT-PCR测定各组大鼠左心室心肌CTGF mRNA水平。结果与假手术组比较,模型组LVEDP、LVMI明显升高,±dp/dt_(max)明显下降,左心室心肌CTGF蛋白及mRNA表达明显升高(P<0.01);与模型组比较,辛伐他汀组、福辛普利组和联合用药组±dp/dt_(max)明显升高,LVMI、LVEDP明显下降,左心室心肌CTGF蛋白及mRNA表达明显下降,其中联合用药组表达水平最低(P<0.01)。结论辛伐他汀与福辛普利单用及联合应用均可有效改善心功能、消退心肌肥厚,联合用药对心肌纤维化抑制作用优于单独用药。Objective To study the effect of simvastatin or fosinopril alone and their combination on myocardial connective tissue growth factor（CTGF） in rats with CHF. Methods A CHF model of rat was established by constricting the abdominal aorta superior to renal artery. Fifty male Wistar rats were randomly divided into sham operation group, model group, simvastatin group, fosinopril group, and combination group, 10 in each group. Their LVMI, left ventricle dilation pressure（LEVDP）, maximium LVEDP and its decreasing rate（tdp/dt ） were observed. Expression of CTGF protein in left ventricle of rats was detected by SP immunohistochemistry. Expression levels of CTGF protein and mRNA in left ventricle of rats were measured by Western blot and RT-PCR, respectively. Results The LVMI and LVEDP and the expression levels of CTGF protein and mRNA were significantly higher while the ±dp/dt was significantly lower in model group than in sham operation. The± dp/dt was significantly higher while the LVMI and LVEDP and the expression levels of CTGF protein and mRNA were significantly lower in simvastatin group,fosinopril group and their combination group than in model group（P〈0. 01）. Conclusion Simvastatin or fosinopril alone and their combination can improve the cardiac function and myocardial hypertrophy in rats with CHF. However, the inhibitory effect of combined simvastatin and fosinopril on myocardial fibrosis is better than that of simvastatin or fosinopril alone.

关 键 词：心力衰竭 结缔组织 心室功能 左 福辛普利 肥大 左心室 心室复建 RNA 信使 

分 类 号：R541.6[医药卫生—心血管疾病]