γ-氨基丁酸信号在JAR细胞中的表达及对细胞侵袭能力的影响  

Expression and its significance of GABA signal in gestational trophoblastic cell pathological tissues

作　　者：罗文萍[1] 刘真真[1] 彭洪英[1] 谭冬梅[1] 赵海[1] 谭毅[1] 

机构地区：[1]重庆医科大学实验动物中心,重庆400016

出　　处：《中国临床药理学与治疗学》2012年第3期274-279,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金面上项目(30270510;31171436);重庆市教委项目(KJ110316)

摘　　要：目的:本文通过研究γ-氨基丁酸(GABA)及其受体GABRP、GABAB1R在滋养层细胞株JAR中的表达,采用Transwell模型,给予GABA受体的激动剂和拮抗剂干预,探讨其对JAR细胞侵袭能力的影响。方法:免疫细胞化学,RT-PCR,Western blot方法检测GABA、GABRP、GABAB1R滋养层细胞株JAR中的表达;Matri-gel细胞侵袭模型检测GABA、GABAAR,GAB-ABR激动剂和拮抗剂对JAR细胞侵袭能力的影响。结果:GABA、GABRP、GABAB1R在细胞株JAR均有表达;GABAA受体激动剂促进JAR细胞的侵袭,而GABAB受体激动剂抑制JAR细胞的侵袭。结论:在滋养层细胞株JAR中,GA-BA信号可能调节细胞的侵袭能力,从而参与滋养细胞相关妊娠疾病的发生发展。To explore the effects of GABAR agonist or antagonist on JAR cells inva sion by identifying the expression of GABA, re subunit of GABARA, B1 subunit of GABARB （GABABIR） in JAR cells and taking Transwell chamber with GABA or receptor agonist or an- tagonists. METHODS： The protein expression of GABA, GABRP, GABABIR were measured by immunocytochemistry and western bolt in JAR cells. The mRNA GABRP, GABAB1R was measured by RT-PCR. The effects of GABA and its receptors agonists and antagonists on JAR cells invasion were assay by Transwell chamber.RESULTS. GABA, GABRP, GABABIR were expressed in JAR cells. GABAa receptor agonist muscimol significantly promoted the invasion of JAR cells, while GABA^R agonist baclofen sig- nificantly inhibited JAR cell migration. CON- CLUSION= GABA signal may be associated with the pathogenesis of trophoblast-associated disea- ses of pregnancy.

关 键 词：JAR细胞 Γ-氨基丁酸 侵袭 

分 类 号：R965.2[医药卫生—药理学]