An Improved Method for DNA Extraction from the Faeces of Red Deer  

一种从马鹿粪便中提取DNA的改进方法(英文)

作　　者：日沙来提·吐尔地[1] 艾斯卡尔·买买提[1] 日孜汗·阿布地艾尼[1] 阿米拉·阿布来提[1] 马合木提·哈力克[1] 

机构地区：[1]新疆大学生命科学与技术学院资源生物研究所,新疆乌鲁木齐830046

出　　处：《Agricultural Science & Technology》2012年第4期698-700,900,共4页农业科学与技术（英文版）

基　　金：Supported by the National Natural Science Foundation of China(31060152);the Natural Science Foundation of the Xinjiang Uygur Autonomous Region,China(2010211A02);the Key Program for Animal Sciences of Xinjiang University,China~~

摘　　要：[Objective] To introduce an improved method for DNA extraction from the faeces of red deer. [Method] Based on the traditional method of CTAB lysis, we proposed an improved DNA extraction method according to the characteristics of red deer faeces. [Result] This improved method extracted high-quality fecal DNA from Tianshan red deer and amplified the mitochondrial cytochrome b gene. With the muscle and fur DNA of red deer as the control, the sequencing results further con- firmed the reliability of the method. [Conclusion] The method requires no proteinase K in the process of extraction, and the extracted DNA can be used for PCR ampli- fication directly without the purification of DNA purification kit, thus, it is cost-saving.[目的]介绍一个从马鹿粪便中提取DNA的改进方法。[方法]本实验采用的是,在传统的CTAB裂解法的基础下,根据马鹿粪便的特征摸索出来的改进方法。[结果]采用该方法从天山马鹿粪便中提取了高质量的粪便DNA并扩增了天山马鹿线粒体细胞色素b基因片段,通过测序检测,同时提取马鹿肌肉和皮毛样品的DNA作为对照,进一步证实了提取的可靠性。[结论]该方法提取过程中不需要用蛋白酶K;所提取的DNA不需要用DNA纯化试剂盒纯化,可直接用PCR扩增,因此,费用量很低。

关 键 词：Red deer Noninvasive sampling Improved method for DNA extraction Faecal DNA 

分 类 号：S829.9[农业科学—畜牧学]