槐白皮水提物对^(60)Co-γ射线损伤小鼠脾细胞的免疫保护作用研究  

Study on the Immune Protective Effect of Sophora japonical Water Extract on the Spleen Cells of ^(60)Co-γ Ray Irradiated Injured Mice

作　　者：张跃进[1] 董海艳[2] 张婵[2] 李安乐[3] 陈向敏[2] 

机构地区：[1]温州医学院内基实验室,浙江温州325035 [2]温州医学院生物学实验教学中心,浙江温州325035 [3]温州医学院实验动物中心,浙江温州325035

出　　处：《中国药房》2012年第15期1354-1355,共2页China Pharmacy

基　　金：浙江省教育厅科研计划项目(20030800)

摘　　要：目的:研究槐白皮水提物对^(60)Co-γ射线损伤小鼠脾细胞的免疫保护作用。方法:实验分为5组,即正常(等容生理盐水)、照射对照(等容生理盐水)和槐白皮水提物高、中、低剂量(0.8、0.4、0.2mL.kg-1)组。除正常组外其余各组给予^(60)Co-γ射线一次性照射,照射后立即给药,每天1次,连续10d。测定小鼠脾细胞培养上清液中一氧化氮(NO)水平及脾指数。结果:槐白皮水提物中剂量组NO含量显著低于照射对照组(P<0.05);槐白皮水提物中、低剂量组脾指数显著高于照射对照组(P<0.01或P<0.05)。结论:槐白皮水提物能降低射线对脾细胞的损伤,具有免疫保护作用。OBJECTIVE：To evaluate the immune protective effect of Sophora japonical water extract on the spleen cells of ^（60）Co-γ ray irradiated injured mice.METHODS：Mice were randomly divided into 5 groups：normal group（constant volume normal saline）,irradiation control group（constant volume normal saline）,S.japonical water extract high-dose,medium-dose and low-dose groups（0.8,0.4,and 0.2 mL·kg-1）.All the mice were irradiated with ^（60）Co-γ ray except normal group,and then S.japonical water extract was given once a day for 10 days.And then nitric oxide（NO）level and spleen index was detected in spleen cell of mice.RESULTS：NO level of S.japonical water extract medium-dose group was significantly lower than irradiation control group（P0.01）,and the spleen index of S.japonical water extract medium-doce group and low-dose groups was significantly higher than irradiation control group（P0.01 or P0.05）.CONCLUSIONS：S.japonical water extract can reduce the damage of the irradiated spleen cells,and have the immune protective function.

关 键 词：槐白皮 一氧化氮 脾指数 放射损伤 

分 类 号：R281.4[医药卫生—中药学] R282[医药卫生—中医学]