南蛇藤总萜对Hepa1-6荷瘤小鼠VEGF、CECs的影响  

The effects of COE on the expression of VEGF and CECs of Hepa1-6 tumor bearing mice

作　　者：侯莹[1] 聂春兰[1] 刘延庆 

机构地区：[1]江苏省溧水县人民医院肿瘤科,211200 [2]扬州大学中西医结合研究所,225009

出　　处：《国际中医中药杂志》2012年第4期324-326,共3页International Journal of Traditional Chinese Medicine

摘　　要：目的观察南蛇藤总萜对Hepa1-6荷瘤小鼠外周血VEGF、CECs的影响。方法采用常规无菌接种技术制作小鼠肝癌Hepa1-6移植性肿瘤模型，观察南蛇藤总萜高、中、低剂量（40mg／kg、20mg／kg、10mg／kg）对Hepa1-6荷瘤小鼠外周血VEGF、CECs表达的影响。结果南蛇藤总萜在20rng／kg时，外周血vEGF（45．24±21．36）pg／ml、CECs（0．83±0．10）％表达明显降低，与阴性对照组（0．9％生理盐水）比较，差异有统计学意义（P〈0．01）。结论南蛇藤总萜可明显降低VEGF、CECs表达，这一作用可能与南蛇藤总萜抑制肝癌血管生成有关。Objective To study the effects ofCelastrus orbiculatus extracts （COE） on the expression of VEGF and CECs of Hepal-6 tumor bearing mice. Methods Animal Models of Hepal-6 portability liver tumor bearing mice were established and randomly assigned to six groups （eight mice per group） as follows： untreated control group, solvent vehicle control （DMSO） group, physiological saline control group, cisplatin-treated group, and different dosages COE-treated groups （40 rag＆g, 20 mg/kg, 10 mg/kg） , To approach the effects of COE on the expression of VEGF and CECs in Hepal-6 toumor bearing mice. Results The ELISA and flow cytometer showed that COE could significantly inhibit the expression of VEGF（45.24± 21.36） p g/ml and CECs （0.83±0.10） % compared with the physiological saline control group （P 〈 0.01 ） with the dose of 20mg/kg. Conclusion COE could significantly down-regulate the expression of VEGF and CECs. It may be related with the effect of COE in inhibiting angiogenesis of tumor.

关 键 词：南蛇藤 血管生成 循环血管内皮细胞 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]