腺病毒表达鸡GM-CSF及其对鸡骨髓细胞增殖活性的检测  

Adenovirus expression and activity test of chicken GM-CSF

作　　者：王兴龙[1] 郝华芳[1] 杜恩岐[1] 唐文雅[1] 张鹏[1] 陈胜利[1] 张渭东[1] 张淑霞[1] 党如意[1] 杨增岐[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《中国兽医学报》2012年第4期509-512,共4页Chinese Journal of Veterinary Science

基　　金："十一五"国家科技支撑计划资助项目(2007-2010)

摘　　要：为了方便鸡粒细胞/巨噬细胞集落细胞刺激因子(GM-CSF)作为疫苗佐剂使用,用PCR方法扩增出鸡GM-CSF基因,将其连入人腺病毒穿梭载体pAdTrack-CMV,将重组穿梭载体与腺病毒骨架重组后,获得重组腺病毒骨架载体,线性化后转染293SD细胞,获得能够表达鸡GM-CSF的腺病毒。将获得的重组腺病毒转导鸡成纤维细胞系DF1,细胞培养上清中,可以检测到GM-CSF刺激鸡骨髓细胞活性,为鸡GM-CSF进一步应用奠定了基础。To conveniently use chicken GM-CSF as cytokines adjuvant in chicken vaccine,chicken GM-CSF were amplified and cloned into adenovirus shuttle vector.After recombinant with the linearization shuttle vector,the recombinant plasmid containing chicken GM-CSF was used to transfect 293SD cells.The recombinant adenovirus containing chicken GM-CSF was recovered from the 293SD cells.The recombinant adenovirus were transducted into chick embryo fibroblast cells（cell line DF1）,in the culture medium,the activity of chicken GM-CSF could be detected.The recombinant adenovirus may be a useful candidate for improve chicken vaccine effect.

关 键 词：鸡GM-CSF 重组腺病毒 活性检测 

分 类 号：S852.65[农业科学—基础兽医学]