谷胱甘肽对铜孵育的肉鸡肝细胞线粒体的保护作用  

Protective effect of glutathione on copper exposure to liver mitochondria of broilers

作　　者：苏荣胜[1] 闫文龙[2] 潘家强[1] 贾雪霞[1] 李海琴[1] 许冬蕾[1] 唐兆新[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]韶关学院英东生物工程学院,广东韶关512005

出　　处：《中国兽医学报》2012年第4期602-605,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30871900);广东省自然科学基金资助项目(8451200501000533)

摘　　要：向即时分离的肉鸡肝细胞线粒体培养液内加入终浓度分别为10、30、50μmol/L的Cu2+和200μmol/L GSH+50μmol/L Cu2+,孵育20min后,观察培养液中不同铜浓度对线粒体H2O2生成速率和呼吸链复合物活性的影响。结果表明,30μmol/L和50μmol/L的Cu2+能引起线粒体H2O2生成速率和呼吸链复合物活性出现明显加快和降低(P<0.05或P<0.01),10μmol/L Cu2+组没有出现明显变化;谷胱甘肽可显著抑制50μmol/L Cu2+引起的变化(P<0.01),对线粒体具有保护作用。Hydrogen peroxide（H2O2） generation and activities of respiratory chain complexes of mitochondria were observed after incubating the mitochondria which was freshly isolated from broiler hepatocyte with 10,30,50 μmol/L Cu2＋ and 200 μmol/L glutathione（GSH）＋50 μmol/L Cu2＋for 20 min,respectively.The values of H2O2 generation and activities of respiratory chain complexes advanced and decreased significantly correspondingly（P0.05 or P〈0.01） after Cu2＋ exposure at 30 and 50 μmol/L,and no obvious difference at 10 μmol/L Cu2＋.GSH could obviously inhibit changes of H2O2 generation and activities of respiratory chain complexes after Cu2＋ exposure at 50 μmol/L（P〈0.01） and protect mitochondria.

关 键 词：铜 线粒体 过氧化氢 线粒体复合物 谷胱甘肽 

分 类 号：S852.2[农业科学—基础兽医学]