链霉菌I06A-00625发酵产物的提取分离、结构鉴定及活性研究  

Isolation,purification,structure identification and activity study of products from Streptomyces I06A-00625

作　　者：贾贝[1] 何宁[1] 赵莉莉[1] 余利岩[1] 蒋忠科[1] 姜蓉[1] 

机构地区：[1]中国医学科学院北京协和医学院医药生物技术研究所微生物化学室,北京100050

出　　处：《中国医药生物技术》2012年第2期116-119,共4页Chinese Medicinal Biotechnology

基　　金：创新微生物药物高效筛选与发现技术平台(2009ZX09302-004)

摘　　要：目的从链霉菌I06A-00625(cpcc201504)的代谢产物中分离得到具有抑菌活性的抗生素,并进行结构鉴定。方法通过大孔吸附树脂X-5、葡聚糖凝胶LH-20和HPLC色谱对发酵液中活性成分进行分离纯化;根据紫外、高分辨质谱并结合1H-NMR和13C-NMR数据确定化合物结构,并进行抗菌活性研究。结果分离纯化得到6个单一组分,分别为化合物I06A625A、206A和206B及3个四烯大环内酯类抗生素Tetramycin A、Tetramycin B和Tetrin A。其中206A为首次从天然产物中分离得到。I06A625A具有抗铜绿色假单胞菌活性;Tetramycin A、Tetramycin B和Tetrin A具有抗真菌活性。结论链霉菌I06A-00625可产生抗铜绿色假单胞菌的核苷类化合物及抗真菌的四烯类化合物等多种活性成分。Objective To isolate and purify antibiotics from the metabolites of streptomyces I06A-00625 （cpcc201504）, and determine its structure and activity. Methods Macroporous resin X-5, Sephadex LH-20 and HPLC were used to isolate and purify compounds with antifungal activity. Structures of the compounds were determined by UV, HR-MS and NMR spectral data. Their antifimgal activity was also studied. Results Total six compounds were finally obtained, including I06A625A, 206A, 206B and three tetraene macrolide antibiotics, Tetramycin A, Terramycin B and Tetrin A. Among them, 206A was first isolated from natural product as reported. Activity study showed that I06A625A had anti-aeruginous Pseudomonas activity, and three tetraene macrolide antibiotics had anti-fungal activity. Conclusions Streptomyces I06A-00625 could produce a variety of active compounds including nucleoside with the anti-aeruginous Pseudomonas activity and tetraene macrolide antibiotics with anti-fungal activity.

关 键 词：链霉菌属 抗生素类 抗真菌 发酵 

分 类 号：R915[医药卫生—微生物与生化药学]