异基因造血干细胞移植受者CMV检测方法的比较  

Analysis of different methods for detecting human cytomegalovirus infection in allogeneic hematopoietic stem cell transplantation recipients

作　　者：郝新建[1] 周健 房佰俊 张莉 符粤文 魏旭东 宋永平 

机构地区：[1]郑州大学第一附属医院血液科,河南郑州450052 [2]郑州大学附属肿瘤医院血液科,河南郑州450008

出　　处：《河南医学研究》2012年第1期15-17,共3页Henan Medical Research

基　　金：国家自然科学基金项目(30900637)

摘　　要：目的:比较异基因造血干细胞移植(allo-HSCT)后早期有效检测巨细胞病毒(CMV)激活感染的方法。方法:分别应用荧光定量PCR和ELISA试剂盒检测57名allo-HSCT受者642份标本的血浆DNA负荷量和血清IgM抗体。结果:IgM抗体和DNA定量的阳性检出率为35.51%(228/642)和13.08%(84/642),连续阳性病例和临床诊断的符合率分别为33.33%(9/24)和87.5%(21/24),IgM抗体阳性检出率明显低于DNA定量,差异有统计学意义(P<0.05)。结论:荧光定量PCR检测allo-HSCT受者CMV早期感染可靠、简便快速,值得临床推广使用。Objective： To search the better detection method of cytomegalovirus（CMV） in allogeneic hematopoietic stem cell transplantation（allo-HSCT） recipients. Methods： All 642 clinical specimens from allo-HSCT 57 patients were examined with the real-time（FQ-PCR） to assay plasma DNA and ELISA to measure the serum level of IgM antibody against CMV. Results： The positive rates of IgM antibody and DNA load were 13.08%（84/642） and 35.51%（228/642）,the corresponding rates between their sequential detection rates and clinical diagnosis were and 9/24 21/24.The positive rate of IgM antibody detected by ELISA is obvious lower than that of DNA quantitated by FQ-PCR.The difference has shown statistical significance（P〈0.05）. Conclusion： FQ-PCR is a sensitive,rapid,suitable and reliable method for monitoring allo-HSCT recipients of incipient reactive CMV infection and is worthy to extensively use for guiding antiviral therapy.

关 键 词：巨细胞病毒感染 检测 异基因造血干细胞移植 

分 类 号：R55[医药卫生—血液循环系统疾病]