β1-肾上腺素受体自身抗体对大鼠B淋巴细胞增殖的影响  

The influence on the proliferation of rat B lymphocytes induced by β1-adrenoceptor autoantibodies

作　　者：吕婷婷[1] 杜芸辉[2] 王瑾[1] 李晓宇[1] 王瑞[1] 刘慧荣[1,2] 

机构地区：[1]山西医科大学生理学系,太原030001 [2]首都医科大学生理与病理生理学系,100069

出　　处：《中华微生物学和免疫学杂志》2012年第2期97-101,共5页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：国家自然科学基金资助项目（81070263）;山西医科大学细胞生理学省部共建重点实验室主任基金（201006）

摘　　要：目的探讨β1-肾上腺素受体自身抗体（β1-AA）对大鼠B淋巴细胞增殖的影响。方法本研究以主动免疫大鼠的方法获得β1-AA阳性的IgGs（pIgGs）；免疫磁珠分选技术获得大鼠B淋巴细胞；CCK8法检测B淋巴细胞的增殖能力。结果β1-AA（0．1μmol／LpIgGs）促进LPS刺激的大鼠B淋巴细胞增殖（A值：0．739±0．036VS0．533±0．032，P〈0．05），且有浓度依赖性的趋势；该效应可以分别被β1／β2-肾上腺素受体（β1／β2-AR）阻断剂部分阻断，被两种阻断剂共同作用完全阻断；pIgGs对静息的大鼠B淋巴细胞无增殖作用。结论β1-AA可能通过B淋巴细胞表面的β1-AR和／或β2-AR信号通路促进激活的B淋巴细胞增殖。Objective To investigate the influence of autoantibodies against the second extracellular loop of β1 -adrenoceptor （ β1 -AA） on the proliferation ability of LPS-stimulated rat B lymphoeytes. Methotis Active immunization assay was used to obtain adequate IgGs in which β1-AA was positive; MACS （magnetic activated cell sorting） assay was used for gaining rat splenic B lymphocytes; CCK8 assay was used for detecting the influence of β1 -AA to the proliferation abilities of silent and LPS-stimulated rat splenic B lymphocytes. Results β1-AA （0. 1 μmol/L pIgGs） promoted the proliferation of LPS-stimulated rat splenic B lymphoeytes（A values ：0. 739±0.036 vs 0.533±0. 032, P〈0.05） , and the effect was likely to be concentration-dependent. And the effect could be blocked by β1-AR blocker or β2-AR blocker partially, and could be blocked by β1-AR blocker and β2-AR blocker completely; β1-AA had no proliferation effect on si- lent rat splenic B lymphocytes. Conclusion β1-AA could promote the proliferation of LPS-stimulated rat splenic B lymphocytes via β1-AR and β2-AR which were on the surface of B lymphocytes. Thus, it could provide new clues for complex pathologic mechanism of cardiovascular patients in which β1-AA is positive.

关 键 词：Β1-肾上腺素受体 自身抗体 B淋巴细胞 增殖 

分 类 号：R392.1[医药卫生—免疫学]