E型沙眼衣原体重组主要外膜蛋白诱导恒河猴的交叉免疫效应  

The cross immune responses of the rhesus monkey induced by the E style Chlamydia trachomatis re- combinant major outer membrane protein

作　　者：李宜儒[1] 姚卫锋[1] 展小飞[1] 王敬[1] 盛彩虹[1] 李艳飞[1] 冯斌[1] 刘原君[1] 齐蔓莉[1] 刘全忠[1] 

机构地区：[1]天津医科大学总医院皮肤科／天津皮肤性病研究所, 300052

出　　处：《中华微生物学和免疫学杂志》2012年第2期161-165,共5页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：国家自然科学基金项目（30671879）

摘　　要：目的探讨E型重组主要外膜蛋白（rMOMP）对恒河猴诱导产生的衣原体交叉免疫应答效应。方法恒河猴分3组，每组2只，分别为佐剂蛋白组、佐剂组、对照组。于第0、2、4周双侧肱三头肌注射。末次免疫后两周，ELISA检测恒河猴血清中沙眼衣原体特异性IgG抗体和细胞因子IFN-γ，MTT法检测恒河猴淋巴细胞特异性增殖反应，观察恒河猴的迟发型超敏反应，以及恒河猴的血清抗体中和试验。结果佐剂蛋白组产生了较强的抗rMOMP反应和高水平细胞因子。淋巴细胞特异性增殖反应、迟发型超敏反应明显强于对照组，抗体中和试验蛋白佐剂组血清能抑制D／E／H／L2型沙眼衣原体生长。结论沙眼衣原体rMOMP能刺激恒河猴产生有效的交叉免疫。Objective To test cross immune responses induced in rhesus monkeys immunized with the recombinant major outer membrane protein（rMOMP）. Methods Six rhesus monkeys were divided into three groups： the group vaccinated with purified rMOMP and Freund＇s adjutants, the group vaccinated with Freund＇s adjutants only and the control group vaccinated with PBS. All of the rhesus monkeys vaccinated in- tramuscularly at 0, 2, 4 weeks. Two weeks after the last time, The IFN-γ and Chlamydia-specific antibody titers in sera, which were determined by ELISA, lymphocyte proliferation assay were performed by MTT, and observ the delayed hypersensitivity and in vitro neutralization assays. Results The result of the monkeys immunized with rMOMP and Freund＇s adjuvant：the specific immune responses can be observed. The in vitro neutralization and lymphocyte proliferation assays were observed better in the same group. Conclusion Af- ter being vaccinated with rMOMP, the monkeys can develop strong and effective Chlamydia-specific cross immune responses.

关 键 词：沙眼衣原体 重组主要外膜蛋白 疫苗 交叉免疫 恒河猴 

分 类 号：R392[医药卫生—免疫学]