腺病毒5型和7型感染诱导呼吸道上皮细胞黏蛋白1表达的差异性研究  

Differences of the regulation on the expression of mucin 1 （ MUC1 ） induced by adenovirus serotype 5 and serotype 7 infections in airway epithelial cells

作　　者：张梦雯[1] 倪淑媛[2] 李煜生[3] 

机构地区：[1]南方医科大学第一临床医学院,广州510515 [2]南方医院急诊科,广州510515 [3]南方医科大学基础医学院病理生理学教研室,广东省蛋白质组学重点实验室,教育部重大疾病的转录组与蛋白组学重点实验室,广州510515

出　　处：《中华微生物学和免疫学杂志》2012年第2期172-177,共6页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：国家自然科学基金青年项目（81100008）;广东省高校优秀青年创新人才培育项目（LYM10048）

摘　　要：目的为探讨人类呼吸道腺病毒感染自限性的分子机制，对人类常见呼吸道腺病毒5型（AdS）和7型（Ad7）感染对呼吸道上皮细胞抑炎分子——黏蛋白1（MUC1）表达的影响进行初步研究，并探讨两者差异性。方法分别用Ad7和AdS感染呼吸道上皮细胞A549构建感染模型。qRT-PCR和Westernblot分别检测感染后MUC1mRNA转录水平及蛋白表达水平变化。结果Ad5感染A549细胞后MUCImRNA转录水平及MUC1蛋白表达水平均可见上调，且呈一定的时间效应；而Ad7感染后未观察到此现象，延长Ad7感染时间后，仍未观察到MUC1mRNA转录上调。结论人类呼吸道AdS感染呼吸道上皮细胞初期，可诱导上调抑炎分子MUC1表达，提示MUC1全部或部分参与到Ad5感染的自限性过程。但Ad7感染不能诱导上调MUC1表达，其感染的自限性来源于其他机制。Objective To explore the molecular mechanism for the self-limitation of adenoviral in- fections in human airway, the different impacts of adenovirus serotype 5 （ AdS ） and serotype 7 （ Ad7 ） infections on ~nucin 1 （ MUC1 ） expression in airway epithelial cells were preliminarily investigated. Methods The AdS and the Ad7 infection models were established in A549 cell line. qRT-PCR was performed to deter- mine the transcription of MUCI mRNA, and the expression of MUC1 in A549 cells infected by AdS or Ad7 was by detected Western blot. Results An up-regulation of the MUC1 mRNA level were observed after AdS infection for 6 h（P〈0.05 ）, and the protein expression level of MUC1 increased in a time-dependent manner in 48 hours of AdS infection , while similar response of MUCI mRNA was absent in Ad7 infection （6 h）, even after prolonged （ 20 h ） treatment （ P 〉 0.05 ）. Conclusion This study reveals an up-regulation of MUCI expression as one of the early immune response to AdS infection, which implies that MUC1 may func- tion fully or partially as an anti-inflammatory factor in the self-limitation effect of AdS infection. However, type7 adenoviral infection, may introduce a mechanism otherwise, but through MUC1.

关 键 词：腺病毒5型 腺病毒7型 呼吸道上皮细胞 黏蛋白1 炎症调控 

分 类 号：R5[医药卫生—内科学]