液相色谱串联质谱法测定人体血浆维拉帕米浓度及生物等效性评价研究  

LC-MS/MS Determination of Verapamil in Human Plasma and Its Application in Bioequivalence Evaluation

作　　者：陈尧[1] 谭志荣[1] 王医成[1] 邓晓兰[1] 张伟[1] 周宏灏[1] 

机构地区：[1]中南大学临床药理研究所 湖南省遗传药理学重点实验室,长沙410078

出　　处：《中国药学杂志》2012年第7期546-550,共5页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(200830801421);中央高校基本科研业务青年教师助推项目(201012200047)

摘　　要：目的建立LC-MS/MS测定人体血浆中维拉帕米的浓度。方法色谱柱为Thermo Hypurity C18(2.1 mm×150 mm,5μm),流动相为10 mmol.L-1甲酸铵(含0.1%甲酸)-乙腈(35∶65);流速:0.3 mL.min-1;进样量:10μL;柱温:40℃,样品室温度为15℃。ESI源正离子扫描,维拉帕米和内标丙咪嗪离子监测分别为(m/z)455.0→165.0和(m/z)281.0→86.0。结果维拉帕米线性范围为0.457 5～234.20 ng.mL-1,维拉帕米最低检测限为0.1 ng.mL-1,方法灵敏、稳定、特异性高,并成功地应用到人体维拉帕米药动学的研究。结论该方法简便、准确、重复性好,可以准确地定量人体血浆维拉帕米的浓度。OBJECTIVE To establish a LC-MS/MS method for determination of verapamil in human plasma METHODS Thermo Hypurity C18 column （2. 1 mm ×150 mm, 5 p,m） was used in the experiment. The column temperature was set at 40 ℃. 10 mmol . L -1 ammonium （ including 0. 1% ammonium formate） -acetonitrile （ 35： 65 ） was used as the mobile phase and the flow rate was 0. 3 mL . min-1. The sample room temperature was set at 15 ℃ and the injection volume was 10 uL ESI source and positive ion scan were used, verapamil and the internal standard imipramine were monitored at （m/z） 455.0-165.0 and （m/z） 281.0-86.0, respectively. RESULTS The calibration curve of verapamil was linear in the range of 0. 457 5 - 234. 20 ng . mL - 1 , and the detection limit for verapamil was about 0. 1 ng . mL-1. The method had high sensitivity, stability and specificity and had been used for the pharmacokinetic study of verapamil in vivo successfully. CONCLUSION The method is simple, accurate and repetitive for the determination of verapamil in human plasma and suitable for pharmacokinetic study.

关 键 词：维拉帕米 液相色谱质谱联用 药动学 

分 类 号：R969[医药卫生—药理学]