三白草中化学成分对H_2O_2损伤LO2细胞保护作用  

Research on the effect of contents from Saururus chinensis on H_2O_2-induced LO2 Cell Damage

作　　者：徐春蕾[1] 李祥[1] 陈宏降[1] 薛平[1] 吴玉兰[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210046

出　　处：《南京中医药大学学报》2012年第2期163-164,共2页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：南京中医药大学青年自然科学基金项目(11XZR11);江苏省普通高校研究生科技创新计划(794);江苏高校优势学科建设工程资助项目(ysxk-2010)

摘　　要：目的研究三白草中主要化学成分对H2O2损伤人正常肝细胞LO2的保护作用。方法供试品为三白草中木脂素类成分:Rel-(7S,8R,7’R,8’R)-3,3’,4,4’,5,5’-hexamethoxy-7-O-7’,8,8’-llignan、Di-O-methyltetra hydrofuriguaiacin B、里卡灵A、里卡灵B、三白草素、二氢愈创木酸;黄酮类成分:异槲皮苷、槲皮苷。LO2细胞接种于96孔板培养,加入100μmol/L的受试成分,培养24h后加入20μL 1.2mmol/L H2O2作用1h。使用MTT法检测各组细胞存活率。结果异槲皮苷和槲皮苷对H2O2损伤LO2细胞有显著保护作用,里卡灵A和二氢愈创木酸对细胞有显著损伤作用。结论该实验可以为三白草保肝作用的研究提供理论基础。OBJECTIVE To study the protective effect of major contents from saururus chinensis on H2O2-induced LO2 cells damage.METHODS Tested samples are lignanoids： Rel-（7S,8R,7＇R,8＇R）-3,3＇,4,4＇,5,5＇-hexamethoxy-7-O-7＇,8,8＇-llignan、Di-O-methyltetra hydrofuriguaiacin B、Licarin A、Licarin B、Saucernetin and Dihydroguaiaretic acid;flavonoids ： Isoquercitrin and Quercitrin.LO2 cells were cultured in 96 well plates.Then 100 μmol/L of tested samples were added.After 24 h,cells were treated with 1.2 mmol/L H2O2 for 4 h.MTT assay was used to detect survival rate of LO2 cells.RESULTS Isoquercitrin and quercitrin had distinguished protective effect,but licarin A and dihydroguaiaretic acid had distinguished damaging effect.CONCLUSION The study can be used as research theoretic basis of liver protection from saururus chinensis.

关 键 词：三白草 LO2细胞 存活率 

分 类 号：R285.5[医药卫生—中药学]