中华眼镜蛇蛇毒组分C诱导白血病细胞凋亡作用的研究  

Study on Human Leukemia Cell Apoptosis inducing Effect of Fraction C of Naja naja Actra Venom

作　　者：郝玉书[1] 应红光[1] 林振桃[2] 管锦霞[2] 张广森[3] 

机构地区：[1]中国医学科学院血液病研究所 [2]广州医学院蛇毒研究所 [3]湖南医科大学第二附属医院

出　　处：《中国中西医结合杂志》2000年第4期272-275,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：广东省卫生厅医学科研基金!A1999249;广东省中医药管理局立项课题! 99583

摘　　要：研究眼镜蛇蛇毒组分Ｃ诱导白血病细胞凋亡的作用及机制。方法：应用光学显微镜、透射电镜、脱氧核糖核酸（ ＤＮＡ）电泳、流式细胞仪、逆转录聚合酶链反应（ＲＴ－ＰＣＲ）等方法，观察白血病细胞经过眼镜蛇蛇毒组分Ｃ处理后，形态学、生物化学及ＢｃＩ－２／Ｂａｘ基因表达水平的变化。结果：眼镜蛇蛇毒组分Ｃ能诱导人粒细胞白血病细胞系（ＨＬ６０）发生凋亡形态学和生物化学变化；流式细胞议分析发现眼镜蛇蛇毒组分Ｃ能使一定比例的人粒单细胞白血病细胞系（Ｊ６－１）、人红白血病细胞系（Ｋ５６２）、ＨＬ６０。细胞及取自患者骨髓的白血病细胞发生凋亡，而且凋亡率随蛇毒浓度的增高而增加；ＲＴ－ＰＣＲ方法检测发现眼镜蛇蛇毒组分Ｃ能使ＨＬ６０细胞Ｂｃｌ－２基因的表达下调，而Ｂａｘ变化不明显。结论：眼镜蛇蛇毒组分Ｃ能诱导白血病细胞发生凋亡，此作用与Ｂｃｌ－２基因的表达下调有关。To study the effect and mechanism of fraction C isolated from Naja naja Actra Venom (FCNNAV) in inducing apoptosis of human leukemia cells. Methods: Light microscope, transmission electron microscope, DNA electrophoresis, flow cytometry and RT-PCR assay were used to observe the changes of human leukemia cells in morphology and biochemistry, and Bcl-2/Bax expression after exposing to FCNNAV. Results: FCNNAV could induce HL60 cells apoptosis demonstrated by the typical morphological and biochemical changes. Flow cytometry showed that J6-1, K562, HL60 and fresh leukemia cells apoptosis could be induced by FCNNAV, and the apoptosis rate was dose-dependent. RT-PCR detection showed the Bcl-2 gene expression of HL60 cells was down-regulated by FCNNAV, whereas the Bax expression was unaffected. Conclusion: FCNNAV could induce apoptosis of human leukemia cells and this effect is related to down-regulation of Bcl-2 gene expression level.

关 键 词：眼镜蛇蛇毒 白血病 细胞凋亡 实验研究 

分 类 号：R285[医药卫生—中药学]