青藤碱对豚鼠单个心室肌细胞膜钠、钙离子通道的阻滞作用  

The Effect of Sinomenine on Sodium,L type Callium current in Guinea Pig Ventricular Myocytes.

作　　者：丁仲如[1] 李庚山[1] 蒋锡嘉[1] 许家俐[1] 王腾[1] 

机构地区：[1]湖北医科大学附属第一医院心内科,湖北武汉430060

出　　处：《中国心脏起搏与心电生理杂志》2000年第1期39-41,共3页Chinese Journal of Cardiac Pacing and Electrophysiology

摘　　要：应用膜片钳全细胞记录技术研究青藤碱 (Sin)对酶解分离的豚鼠单个心室肌细胞膜钠离子电流 (INa)、L型钙电流 (ICa L)的影响。发现 1,5 ,10 μmol/L的Sin分别使INa峰值 (INamax)较用药前下降 2 3.2 %、36 .1%和 6 0 % (n =8,P均 <0 .0 5 ) ;使ICa L峰值 (ICa Lmax)较用药前下降了 12 .8% ,35 .9%和 46 .9% (n =8,P均 <0 .0 1)。Sin使INa及ICa L电流 电压曲线上移 ,但不使其峰值电位偏移。Sin还浓度依赖性减慢钠通道灭活后恢复过程。当刺激频率分别为 0 .5 ,1,2Hz时 ,5 μmol/LSin使INamax较用药前分别下降了 36 .1%、41.5 %和 48.0 % (n =8,P均 <0 .0 1)。结果表明 ,Sin对INa具浓度和频率依赖性阻滞作用 ,可能作用于其失活状态 ;Sin对ICa L具浓度依赖性阻滞作用。这可能为其抗心律失常的重要机制。Whole cell patch clamp technique was used to investigate the effect of sinomenine (Sin) on sodium current (I Na ) and L type callium (I Ca L ) in isolated guinea pig ventricular myocytes.Sin at 1,5,10 μmol/L decreased peak I Na (I Namax ) 23.3%,36.1%,60%,respectively ( n =8, P <0.05);and reduced I Ca Lmax 12.8%,35.9% and 46.4%,respectively ( n =8, P <0.01).Sin shifted the I V curve of I Na and I Ca L without change of their peak current potentials.Sin delayed the recovery time course of I Na from inactive state in dose dependant manner.When the stimulation frequency was 0.5,1,2 Hz,Sin at 5 μmol/L reduced I Namax 36.1%,41.5% and 48.0%,respectively ( n =8, P <0.05).It can be concluded that Sin blocks I Na and I Ca L in a concentration dependant manner and probably inhibits I Na in its inactive state,which may contribute to its antiarrhythmic effect.

关 键 词：青藤碱 膜片钳 钠通道 L型钙通道 心肌细胞 

分 类 号：R331.38[医药卫生—人体生理学]