骨髓增生异常综合征骨髓单个核细胞c-kit受体表达及功能的研究  

Study of the expression and function of c-kit receptor on the bone marrow mononuclear cells of the patients with myelodysplastic syndromes

作　　者：张泓[1] 邵宗鸿[1] 陈桂彬[1] 王立[1] 张益枝[1] 李克[1] 李莉[1] 孙娟[1] 郝玉书[1] 

机构地区：[1]中国医学科学院,中国协和医科大学血液学研究所,血液病医院,天津300020

出　　处：《中华血液学杂志》2000年第4期176-178,共3页Chinese Journal of Hematology

基　　金：卫生部临床学科重点基金!资助项目( 0 0 0 7);中国医学科学院科学基金

摘　　要：目的　了解骨髓增生异常综合征 (MDS)骨髓单个核细胞c kit受体的表达与功能。方法　采用免疫荧光方法测定c kit受体蛋白 (CD117)的表达 ;逆转录 聚合酶链反应方法测定c kitmRNA的表达 ;细胞培养检测c kit受体的功能。结果　CD117表达率正常人为 (3.0 4± 1.49) % ,MDS患者为 (8.5 8± 5 .2 8) % ,两者差异有显著性 (P <0 .0 5 ) ;RA患者为 (5 .12± 2 .13) % ,RAEB/RAEB t患者为 (10 .0 1±5 .0 7) % ,两者差异有显著性 (P <0 .0 5 ) ;MDS继发白血病患者为 (32 .43± 18.16 ) %。MDS患者c kit基因mRNA表达与CD117表达相一致。正常骨髓单个核细胞体外半固体培养 ,在加入造血干细胞因子、白细胞介素 3、红细胞生成素 (SCF +IL 3+Epo)后形成的粒 巨噬细胞集落 (CFU GM)较仅加入IL 3+Epo显著增多 (P <0 .0 5 ) ,红系爆式集落 (BFU E)数量极显著增多 ,且BFU E体积显著增大 (P <0 .0 1)。MDS患者在上述相同条件下CFU GM数量无明显变化 (P >0 .0 5 ) ,与正常对照比较 ,CFU GM和BFU E数量均显著减少 (P <0 .0 5 )。结论　MDS患者骨髓单个核细胞CD117表达明显高于正常对照 ,且与病情进展相关 ;c kitmRNA表达与CD117表达相一致 ;MDS患者骨髓单个核细胞体外培养时 ,SCF协同IL 3、Epo促进造血干 /祖细胞增殖分化能?Objective To determine the expression and function of c kit receptor on bone marrow mononuclear cells(BMMNC) of the patients with myelodysplastic syndromes.Methods c kit protein(CD 117 ) was detected by direct immunofluorescence assay,c kit mRNA by reverse transcriptive polymerase chain reaction(RT PCR) and the function of c kit receptor by cell culture.Results c kit protein expression in MDS was significantly higher than that in control[(8.58±5.28)% vs (3.04±1.49)%,P<0.05].c kit protein expression in RA patients was significantly lower than that in RAEB/RAEB t patients[(5.12±2.13)% vs (10.01±5.07)%,P<0.05].The rate of c kit protein expression was (32.43±18.16)% in AML cases transformed from MDS(t AML).c kit mRNA expression in MDS was correlated with c kit protein expression.Addition of interleukin 3(IL 3),erythropoietin(Epo) and stem cell factor (SCF) to the semi solid culture of BMMNC showed significant stimulating effects on CFU GM and BFU E formation in normal controls,but did not in MDS patients.Conclusion The protein and mRNA expressions of c kit receptor on BMMNC in MDS are higher than those in normal control,and the function of this receptor in MDS is abnormal.

关 键 词：骨髓增生异常综合征 聚合酶链反应 C-KIT基因 

分 类 号：R551.3[医药卫生—血液循环系统疾病]