糖胺聚糖对受刺激内皮细胞组织因子和凝血酶调节蛋白表达的影响  

Effects of holothuria glycosaminoglycan on the expression of tissue factor and thrombomodulin in stimulated endothelial cells

作　　者：李志广[1] 王学锋[1] 王鸿利[1] 郭为民[1] 刘元昉[1] 璩斌[1] 郭雪梅[1] 储海燕[1] 

机构地区：[1]上海第二医科大学附属瑞金医院,上海血液学研究所,200025

出　　处：《中华血液学杂志》2000年第4期201-204,共4页Chinese Journal of Hematology

基　　金：卫生部科学研究基金!资助项目( 98 1 3 10 );胡应洲基金部分资助

摘　　要：目的　研究由玉足海参提取的糖胺聚糖 (GAG)抗血栓形成作用的机制。方法　分别用1mg/L、5mg/L、10mg/L的GAG和 5mg/L肝素与经 1mg/L细菌脂多糖 (LPS)诱导的人脐静脉内皮细胞孵育 ,6h后观察内皮细胞的促凝活性、组织因子 (TF)和凝血酶调节蛋白 (TM)抗原的表达及TF和TMmRNA转录的变化。结果　GAG降低受刺激内皮细胞的促凝活性、TF抗原的表达和TFmRNA的转录 ,升高TM抗原的表达和TMmRNA的转录。结论　下调受刺激内皮细胞TF表达的同时上调TM表达可能是GAG发挥抗血栓形成作用的主要机制之一。Objective To study the mechanism of antithrombotic effects of holothuria glycosaminoglycan(GAG).Methods Endothelial cells from human umbilical vein which were pre induced with 1?mg/L lipopolysaccharide,were treated by GAG(1?mg/L,5?mg/L,and 10?mg/L,respectively)and 5?mg/L heparin as a control for 6 hours.Procoagulant activity(PCA),the expressions of tissue factor(TF) antigen and thrombomodulin(TM) antigen and their mRNA transcriptions were investigated.Results GAG could down regulate the expression of TF antigen and mRNA,up regulate the expression of TM antigen and mRNA,down regulate the PCA of stimulated endothelial cells.Conclusion Down regulating TF expression and up regulating TM expression of stimulated endothelial cells may be one of the mechanisms of GAG antithrombosis.

关 键 词：糖胺聚糖 内皮细胞 组织因子 凝血酶调节蛋白 

分 类 号：R96[医药卫生—药理学]