重症监护室MRSA耐药性及RAPD分析  

Antibiotics resistance and RAPD analysis of methicillin-resistant Staphylococcus aureus isolated from ICU

作　　者：周秀萍[1] 杨长顺[2] 李争鸣[1] 邱宏[1] 

机构地区：[1]怀化医学高等专科学校,湖南怀化418000 [2]湖南省怀化市第一人民医院,湖南怀化418000

出　　处：《中国卫生检验杂志》2012年第3期509-511,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:调查重症监护室耐甲氧西林金黄色葡萄球菌的耐药及分子流行病学情况,为控制感染提供科学依据。方法:ICU分离的金黄色葡萄球菌采用mecA基因检测法确定MRSA,对筛选出的MRSA采用纸片法检测其耐药性,用随机扩增多态性DNA(RAPD)进行分子流行病学分析。结果:MRSA的分离率为58.5%,对多种抗生素均耐药,没有发现耐万古霉素和替考拉宁的菌株。24株MRSA经RAPD分型,均获得稳定的条带,可分为四型,以Ⅱ型为主,共检出15株。结论:ICU分离MRSA菌株具有多重耐药性,应根据药敏试验结果合理选用抗生素进行治疗;通过RAPD分型研究,可了解MRSA的基因型流行特征,为控制感染提供分子流行病学依据。Objective： To investigate the status of antibiotics resistance and the genetic homology of methicillin-resistant staphylococcus aureus in ICU and provide scientific evidence for controlling MRSA infection. Methods： MRSA was identified by mecA PCR.Drug resistance was perfomed using K-B method.The RAPD technique was used to identify the genotypes.Results： The separation rate of MRSA was 58.5%.MRSA was resistant to many drugs.But all strains were susceptible to vancomycin and teicoplanin.All 24 stains of MRSA produced fingerprints by RAPD,and was classified into four genotypes.15 strains wereⅡtype which was the dominant type.Conclusion： The MRSA isolated from ICU has high and multi drug resistance,we should reasonablly choose drugs according to the susceptibility test. RAPD results reveal the special epidemic genotypes of MRSA and this study can provide molecular epidemiological evidence for control of MRSA infection.

关 键 词：重症监护室 耐甲氧西林金黄色葡萄球菌 耐药性 随机扩增DNA多态性分析 

分 类 号：R378.11[医药卫生—病原生物学]