川芎嗪对妊娠高血压综合征患者脐静脉内皮细胞粘附分子表达的抑制作用  

Inhibitory Effects of Ligustrazine on the Expression of Intercellular Cell Adhesion Molecule-1,Vascular Cell Adhesion Molecule-1 in Human Umbilical Vein Endothelial Cells Induced by Preeclamptic Plasma

作　　者：曾素耘[1,2] 樊素璇[1] 蒋小青 

机构地区：[1]南京铁道医学院妇产科教研室,210009 [2]南京军区总医院妇产科

出　　处：《中华妇产科杂志》2000年第5期282-284,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：铁道部科研基金资助(编号:97-J-32)

摘　　要：目的　探讨妊娠高血压综合征 (妊高征 )患者血浆诱导培养的脐静脉内皮细胞 (HUVEC)表面细胞间粘附分子 1(ICAM 1)、血管细胞粘附分子 1(VCAM 1)的表达以及川芎嗪对其的影响。方法　采用胶原酶、胰蛋白酶混合灌注消化法 ,对正常妊娠妇女 (正常妊娠组 )、妊高征患者 (妊高征组 )的HUVEC进行培养 ,待细胞融合成单层后 ,加或不加川芎嗪进行预处理 ,并以正常未妊娠妇女 (对照组 )作为对照。再加入上述 3组血浆的培养液 ,孵育 16h ,应用酶联免疫吸附试验测定 3组HUVEC表面ICAM 1、VCAM 1的表达 [吸光度值 (A) ]。结果　 (1)未加川芎嗪处理 :妊高征组ICAM 1、VCAM 1表达均高于正常妊娠组 (P <0 .0 1) ,吸光度值 (A)分别为 0 .419± 0 .0 97、0 .2 94± 0 .0 5 8及 0 .2 73±0 .0 6 7、0 .187± 0 .0 47;妊高征组VCAM 1的表达也明显高于对照组 (P <0 .0 5 )。 (2 )加川芎嗪预处理 :妊高征组ICAM 1、VCAM 1的表达明显下降 ,吸光度值为 0 .319± 0 .0 75及 0 .2 13± 0 .0 6 2 ;对照组及正常妊娠组VCAM 1的表达也有下降 (P <0 .0 5 )。结论　妊高征患者血浆能诱导HUVEC表面ICAM 1、VCAM 1的表达 ,应用川芎嗪预处理后 ,可抑制ICAM 1、VCAM 1的升高 ,川芎嗪对妊高征的防治有一定作用。Objective To determine whether plasma from preeclamptic women induces expression of intercellular cell adhesion molecule 1 (ICAM 1) and vascular cell adhesion molecule 1 (VCAM 1) on cultured endothelial cells, and the effects of ligustrazine (LTZ) on the expression of ICAM 1、VCAM 1. Methods Human umbilical vein endothelial cells (HUVEC) were isolated by collagenase mixed with trypsin. LTZ was added to HUVEC monolayers grown to confluency on 96 well microplates for 30 minutes. Then HUVEC were incubated with medium containing 20% plasma (volume/volume) either from women with preeclampsia or normotensive pregnancy or nonpregnant women. ICAM 1, VCAM 1 expression was determined after 16 hours of exposure by an enzyme linked immunosobent assay (ELISA) technique performed on the cell monolayer. Results There was a statistically significant increase in ICAM 1, VCAM 1 expression in the endothelial cells exposed to preeclamptic and nonpregnant plasma compared with those exposed to normotensive plasma. Pretreatment with LTZ could reduce the expression of ICAM 1exposed to preeclamptic plasma, and reduce the expression of VCAM 1 exposed to any of the three sources of plasma. Conclusions Preeclamptic plasma induces ICAM 1 and VCAM 1 expression in HUVEC in vitro. Ligustrazine may inhibit the upregulation effects of ICAM 1 and VCAM 1 expression induced by preeclamptic plasma.

关 键 词：妊高征 川芎嗪 内皮 胞间粘附分子-1 血管细胞粘附分子-1 

分 类 号：R714.24[医药卫生—妇产科学]