黄芪皂苷对人胃癌细胞系MKN-74增殖与侵袭的影响  

The anti-proliferation and anti-invasion effect of astragaloside on in human gastric cancer MKN-74 cell line in vitro

作　　者：王湘华 刘小军 蒋艳[2,3,4] 

机构地区：[1]湖南武冈市人民医院,湖南武冈422400 [2]南华大学附属第一医院,湖南衡阳421001 [3]南华大学 [4]南华大学药物药理研究所,湖南衡阳421001

出　　处：《肿瘤药学》2012年第1期31-33,共3页Anti-Tumor Pharmacy

摘　　要：目的探讨黄芪属植物黄芪的根部的一种提取物——黄芪皂苷(astragaloside,AST)在体外对胃癌MKN-74细胞增殖与侵袭力的影响。方法用五个不同剂量(0,2.5,5,10,20μmoL.L-1)的和胃癌MKN-74细胞共培养24、48、72小时,通过MTT法检测黄芪皂苷(AST)对细胞的抑制作用;Transwell侵袭实验检测黄芪皂苷对MKN-74细胞侵袭力的抑制作用。结果 (1)AST在体外能够显著抑制胃癌MKN-74细胞的增殖,2.5μmoL.L-1,5μmoL.L-1,10μmoL.L-1,20μmoL.L-1黄芪皂苷处理72小时生长抑制率分别为4.65%,7.58%,20.73%,30.38%,呈现明显的剂量和时间依赖性。(2)AST显著抑制MKN-74细胞的侵袭力,呈现剂量和时间依赖性。结论 AST在体外对人胃癌MKN-74细胞有显著的抑制细胞增殖作用。Objective To investigate the anti-proliferation and anti-invasion role of astragaloside（AST） on gastric cancer MKN-74 cell line in vitro.Method MKN-74 cells were exposed to different concentrations of AST for 24？h,48？h and 72？h respectively.The proliferation was detected by 4-methyl-teerazolium（MMT）.Transwell assay was applied to detect the inhibitory effect of the AST on MKN-74 cell invasion.Results（1）MTT showed that AST can inhibit the proliferation of MKN-74 cell line significantly,and the growth inhibition rate was 4.65%,7.58%,20.73%,30.38% respectively after treated with the 2.5μmoL·L-1,5μmoL·L-1,10μmoL·L-1,20μmoL·L-1 of AST,depending on dosage and time obviously.（2）The result of Transwell assay made it clear that AST could inhibit the invasion of human gastric cancer cell line MKN-74 in vitro in a dose and time dependent manner.Conclusion AST significantly inhibited the proliferation and invasion of gastric cancer MKN-74 cells in vitro.

关 键 词：黄芪皂苷 MKN-74细胞系 增殖 侵袭 

分 类 号：R979[医药卫生—药品] R735[医药卫生—药学]