益智仁挥发油薄层指纹图谱研究  

Research on Thin-Layer Chromatography Fingerprint of Volatile Oil from Alpinia Oxyphylla Miquel

作　　者：龙兰艳[1] 胡昌江[1] 李文兵[1] 李倩[1] 吴文辉[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137

出　　处：《成都中医药大学学报》2012年第1期58-59,73,共3页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家自然科基面上项目(批准号:30973941)

摘　　要：目的:建立益智仁挥发油薄层指纹图谱,为其鉴别和质量评价提供依据。方法:采用薄层色谱(TLC)方法,建立了益智仁挥发油的薄层指纹图谱。结果:确定益智仁挥发油薄层条件为:预制硅胶G高效薄层板,以石油醚(60℃～90℃)-环己烷-乙酸乙酯(8∶1∶1)为展开剂,点样量为2μL。显色条件为:喷10%硫酸乙醇溶液,于105℃烘5 min,置紫外光灯下(365 nm)观察,得到益智仁挥发油的薄层色谱指纹图谱。结论:所建立的方法具有简便,专属,稳定,直观,可重复的特点,可用于益智仁的鉴别和质量的评价。Objective：To establish thin-layer chromatography（TLC） fingerprint of Volatile Oil from Alpinia oxyphylla Miquel for providing the reference for its identification and quality evaluation.Methods：TLC fingerprint chromatograms were established by TLC fingerprint of Volatile Oil from Alpinia oxyphylla Miquel.Results：Chromatographic conditions were as follows： stationary phanol-precoated HPTLC silica gel 60 plate;developing solvent：petroleum ether（60 ℃-90 ℃）-cyclohexane-ethylacetate（8∶ 1∶ 1）;The optimum dropping amounts was 2 microlitres;derivative reagent：10%H2SO4 ethanolic solution,heating at 105℃ for 5 min and observing the fluorescent chromatogram in a UV cabinet at 365 nm,then establishing TLC fingerprint of Volatile Oil from Alpinia oxyphylla Miquel.Conclusion：Themethod has been proved to be feasible for the identification of this crude drug.

关 键 词：益智仁 挥发油 薄层指纹图谱 

分 类 号：R284.2[医药卫生—中药学] R286.0[医药卫生—中医学]