漆酶固定化载体的最佳制备工艺研究  

Study on the Best Preparation Process of Laccase's Immobilized Carrier

作　　者：张安龙[1] 张佳[1] 景立明[1] 

机构地区：[1]陕西科技大学,陕西西安710021

出　　处：《污染防治技术》2012年第1期16-20,共5页Pollution Control Technology

基　　金：十二五国家项目基金资助(2011BAC11B04);陕西科技大学研究生创新基金资助

摘　　要：采用环氧化PVA载体固定漆酶。探讨了环氧化温度、pH及环氧化时间等条件对载体合成的影响,通过环氧固载量的大小评估环氧化效果。结果表明:对于100 g质量分数为10%的PVA溶液,投加35 ml 25%的戊二醛,可得到交联度适中的PVA凝胶,将凝胶破碎后在pH11～12的条件下与环氧氯丙烷进行环氧化反应,在T=60℃、环氧化时间为6 h、环氧氯丙烷与二甲基亚砜配比为1:1时可得到环氧固载量最大的环氧化PVA载体。用该载体对漆酶进行固定化,得到固定化漆酶对COD和色度的去除率为游离漆酶处理能力的一倍以上。This paper uses epoxidation of PVA carrier to immobilized laccase, discusses the influence conditions on synthesis, such as the epoxidation of temperature, pH and cyclic oxidation time, discusses on the carrier synthesis through epoxy solid load to assess the effect of epoxidation. The results show that, 100 g mass fraction of 10% of PVA solution, dosing 35 m125 % glutaralde- hyde, can get crosslinking degree of moderate PVA gel, the gel happened epoxidation reaction in pH11- 12 conditions after crush- ing, when T = 60℃ , epoxidation time = 6h, epichlorohydrin and dimethyl sulfoxide ratio = 1 ： 1, it can be obtained the largest load of epoxy solid epoxidation carrier. The COD and color removal rates are doubled from immobilized enzyme to free paint enzyme processing capacity by the carrier of immobilized laccase immobilized laccase.

关 键 词：环氧化PVA载体 固定化漆酶 环氧固载量 深度处理 制浆造纸废水 

分 类 号：O636[理学—高分子化学]