木香饮片和新鲜药材DNA片段的克隆及同源性比较分析  

Cloning and homological comparison analysis on DNA fragments of processed pieces and fresh roots of Saussurea lappa

作　　者：唐金凤[1] 聂媛媛[1] 桂柳姿[1] 鲁耀邦[1] 

机构地区：[1]湖南中医药大学药学院,中药药性与药效三级科研实验室,湖南省中药现代化研究实验室,湖南长沙410208

出　　处：《中草药》2012年第4期761-765,共5页Chinese Traditional and Herbal Drugs

基　　金：国家中医药管理局归国留学人员择优资助项目(2006 LHR 19);湖南省中药学重点学科资助项目

摘　　要：目的对木香的饮片和新鲜药材进行DNA片段的比对分析,为木香的鉴定提供研究思路。方法选取木香饮片和新鲜药材,利用CTAB法提取其基因组总DNA,再进行PCR扩增筛选随机引物,找出能同时扩增饮片及新鲜药材基因组总DNA的随机引物,对饮片及新鲜药材基因组总DNA扩增产生的一致性条带进行克隆,得到阳性菌落,经转化子鉴定后测序。对所测序列进行生物信息学分析和同源性比较。结果木香饮片与新鲜药材长度均为794 bp的克隆片段序列相似度达99.75%,长度为1 116 bp的克隆片段的核苷酸水平比对及氨基酸水平比对的相似度均为100%。结论采用分子生物学手段能对木香饮片和新鲜药材进行鉴定。Objective To compare with the DNA fragments of the processed pieces and fresh roots of Saussurea lappa and provide reference for identification.Methods Processed pieces and fresh roots of S.lappa were selected to extract the total DNA using CTAB method and PCR amplification was used to screen the random primers which could simultaneouly amplify total DNA of both the two materials.To clone the consensus bands acquired by amplifing the genomic total DNA of processed pieces and fresh roots of S.lappa and select the positive colony for sequencing after transformation and homological analysis by the biological information sciences.Results Sequences similar degree of processed pieces and fresh roots of S.lappa were 99.75% and 100%,respectively.Conclusion The molecular biology is an effective method to identify the processed pieces and fresh roots of S.lappa.

关 键 词：木香 分子生物学 DNA 克隆 生物信息学 

分 类 号：R282.12[医药卫生—中药学]