高效毛细管电泳测定夏天无药材中延胡索乙素的含量  

Determination of tetrahydropalmatine in Corydalis decumbens using high-performance capillary electrophoresis

作　　者：姜舜尧[1] 宋景政[2] 周明昊[1] 田颂九[2] 

机构地区：[1]浙江省药品检验所,杭州310004 [2]中国药品生物制品检定所,北京100050

出　　处：《中国药学杂志》2000年第5期336-337,共2页Chinese Pharmaceutical Journal

摘　　要：目的 :应用高效毛细管电泳法对夏天无药材中的成分进行分离并测定其有效成分延胡索乙素的含量。方法 :运行缓冲液为CH3 OH 0 .6mol·L-1Tris溶液 (1∶1,用磷酸调pH =6 .0 ) ,另内含 10mmol·L-1庚烷磺酸钠 ;运行电压 2 0kV检测波长 2 0 0nm ;多潘立酮为内标。结果 :延胡索乙素在 0 .0 2 5～ 0 .2 5mg·ml-1范围内对内标峰面积之比成良好线性关系 (r=0 .9992 ) ,平均回收率为 98.4% ,RSD为 2 .6 % (n =5 )。结论 :实验结果表明 ,运用高效毛细管电泳法可将夏天无药材中的有效成分延胡索乙素与其它成分较好的分离 ,并准确测定其含量。OBJECTIVE:To establish a capillary electrophoresis method for the determination of tetrahydropalmatine in Corydalis decumbens (Thunb.)Pers.METHODS:Methanol 0.6 mol·L -1 Tris(1∶1,adjusted pH to 6.0 with H 3PO 4) including 10 mmol·L -1 sodium heptansulfonate was used as running buffer.The running voltage was 20 kV.The detection wavelenglth was set at 200 nm.Domperidone was used as the internal standand.RESULTS:The calibration curve showed good linearity over the range of 0.025～ 0.25 mg·ml -1 ( r =0.9992).The average recovery was 98.4% and the relative standard deviation was 2.6%( n =5).CONCLUSION:This method is accurate,proper and reliable for the determination of tetrahydropalmatine in Corydalis decumbens (Thunb.)Per.

关 键 词：高效毛细管电泳 夏天无 延胡索乙素 原阿片碱 

分 类 号：R284.1[医药卫生—中药学]