靶向大鼠Caveolin-1基因的短发夹RNA表达载体的构建  

Construction of expression vector of short hairpin RNA targeting for caveolin-1

作　　者：岳扬[1] 孙耕耘[1] 尤青海[1] 王楠[1] 邵敏[1] 张丹[1] 

机构地区：[1]安徽医科大学第一附属医院呼吸内科,安徽合肥230022

出　　处：《临床肺科杂志》2012年第5期779-780,共2页Journal of Clinical Pulmonary Medicine

基　　金：国家自然科学基金(编号:81070054)

摘　　要：目的构建靶向大鼠caveolin-1基因的shRNA表达载体。方法化学合成靶向caveolin-1的单核苷酸链,经退火成双链。将退火得到的双链DNA克隆至表达载体pLL3.7中得到重组质粒,经限制性内切酶酶切、DNA测序进行鉴定。结果通过限制性内切酶酶切、DNA测序证实,成功构建大鼠pLL3.7-cav-1表达载体。结论成功构建了靶向大鼠caveolin-1的shRNA表达载体,为以后进行caveolin-1与ALI/ARDS的相关研究奠定了基础。Objective To construct short hairpin RNA expression vector targeting for rat caveolin-1.Methods Chemosynthetic oligos were annealed and then cloned into the pLL3.7 plasmid to generate the recombinant plasmid pLL3.7-cav-1 which was confirmed by restriction endonuclease digestion and DNA sequencing.Result The vector of pLL-3.7-cav-1 was constructed successfully.Conclusion The shor hairpin RNA expression vector targeting for rat caveolin-1 has been constructed successfully,which facilitated to the study on relationship between caveolin-1 and ALI/ARDS.

关 键 词：短发夹RNA 表达载体 小窝蛋白-1 

分 类 号：R563.8[医药卫生—呼吸系统]