鼠疫耶尔森菌感染动物血清标本中10种抗体检测与分析  

Detection and analysis of 10 antibodies in serum samples of animals infected by Yersinia pestis

作　　者：洪文艳[1] 王浩然[1] 郭兆彪[1] 王津[1] 周蕾[1] 杨瑞馥[1] 

机构地区：[1]中国人民解放军军事医学科学院微生物流行病研究所,北京100071

出　　处：《国际检验医学杂志》2012年第5期517-518,共2页International Journal of Laboratory Medicine

基　　金：国家科技支撑计划项目(2009BAK43B31)

摘　　要：目的分析鼠疫耶尔森菌(Y.pestis)感染动物血清标本中鼠疫抗体的分布规律,寻找新的鼠疫血清学辅助诊断靶标。方法采用自制的酶联免疫吸附试验板条检测不同种属的Y.pestis感染动物血清标本(狗血清5例、羊血清5例及黄鼠血清14例)中的10种靶标抗体。结果各靶标抗体在3种不同种属动物血清标本中具有不同的阳性率;抗FI抗体阳性率最高(100.0%),抗YopD抗体的阳性率较高(狗、羊和黄鼠血清标本的阳性率分别为80.0%、100.0%和78.6%),针对LcrV、YPO1089、YPO1613、YPO2131、YPO2118、YPO1435这6个蛋白的抗体在3种血清标本中的阳性率差异较大,抗YPO3827抗体和抗YPO1303抗体在3种血清标本中的阳性率均较低。结论除抗FI抗体外,针对其他抗原的抗体的分布均存在种属差异,抗YopD抗体有可能成为新的Y.pestis感染辅助诊断靶标。Objective To analyze the antibodies spectrum in serum samples of animals infected by Yersinia pestis（Y.pestis） to identify new potential diagnosis target for Y.pestis infection.Methods 10 target antibodies in serum samples of 5 cases of dog,5 cases of sheep and 14 cases of Citellus mongolicus umbratus,all being infected by Y.pesits,were detected by using self-made enzyme linked immunosorbent assay kits.Results The antibody positive rates were different in serum samples of different animals.The positive rate of anti-FI was all 100.0%,and that of anti-YopD-Ab was 100.0% in serum samples of sheep,80.0% of dogs and 78.0% of Citellus mongolicus umbratus.The positive rates of antibodies specific for LcrV,YPO1089,YPO1613,YPO2131,YPO2118 and YPO1435 varied in serum samples of different animals.The positive rates of antibodies specific for YPO3827 and YPO1303 were low in all samples.Conclusion The spectrum of antibodies specific for Y.pestis could be different in different animals infected by Y.pestis.Beside classical anti-FI antibody,the anti-YopAb might be a new potential diagnostic marker for Y.pestis infection.

关 键 词：鼠疫耶尔森氏菌 抗体病毒 多重检测 酶联免疫吸附测定 

分 类 号：R446.6[医药卫生—诊断学]