^(60)Coγ射线照射联合三氧化二砷对人脑胶质瘤SHG44细胞DNA损伤的研究  

Studies of DNA damage effect of ^(60)Co γ rays combined with As_2O_3 on SHG44 human glioma cells

作　　者：黄辉[1] 蒋宏玲[1] 刘芬菊[2] 蔡锐[1] 张荣君[1] 陈秋秋[1] 赵博[1] 刘美莲[1] 

机构地区：[1]桂林医学院附属医院,广西桂林541001 [2]苏州大学放射医学与公共卫生学院,江苏苏州215007

出　　处：《现代中西医结合杂志》2012年第12期1274-1276,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：广西卫生厅自筹经费科研课题项目资助(Z2011185)

摘　　要：目的研究60Coγ射线照射联合As2O3对SHG44细胞的生长抑制作用。方法以SHG44细胞为实验对象,3H-TdR掺入法研究As2O3和照射对SHG44细胞DNA合成率的影响;用单细胞凝胶电泳法,以DNA彗星尾长及尾部DNA百分含量来比较As2O3、照射分别单独作用及它们联合作用对细胞DNA的损伤。结果联合组SHG44细胞存活率低于As2O3(P<0.01);3组均诱导DNA损伤,联合作用组对细胞DNA的损伤程度明显高于照射及As2O3单独作用组。结论电离辐射和As2O3联合应用对SHG44细胞的杀灭作用均强于电离辐射和As2O3单独作用。Objective It is to observe the anti-proliferation effect of 60Co γ rays combined with As2O3 on Suzhou human glioma cells-44（SHG44） in vitro.Methods 3H-TdR incorporation assay was used to observe the influence of As2O3 and 60Co γ rays on DNA synthesis rate.Single-cell gel electrophoresis assay（SCGE） was used to detect SHG44 cells DNA damage.The index of DNA damage,tail moment and percentage of tail of the comet were used to evaluate the extent of DNA damage.Results SHG44 cells survival rate of combine group was significantly lower than that of group treated with radiation or As2O3 only（P0.01）.DNA damages of SHG44 cells induced with 60Co γ rays,As2O3 or 60Co γ rays combined with As2O3 were significantly increased,and the DNA damage effect of combine group on SHG44 cells was stronger than that of radiation or As2O3 only.Conclusion The killing effect of 60Co γ rays combined with As2O3 on SHG44 cells is stronger than that of 60Co γ rays or As2O3 only.

关 键 词：60Coγ射线 三氧化二砷 DNA损伤 人脑胶质瘤SHG44细胞 

分 类 号：R453[医药卫生—治疗学] R815[医药卫生—临床医学]