槟榔碱上调高果糖诱导胰岛素抵抗大鼠骨骼肌GLUT-4和p-PI3K的表达  

Arecoline Up-regulated the Expression of GLUT4 and p-PI3K of Skeletal Muscle in High Fructose Induced Insulin Resistant Rats

作　　者：陈梦华[1,2] 凌宏艳[1] 周寿红[1] 王光[1] 李兴[1,3] 胡弼[1] 

机构地区：[1]南华大学生理学教研室,湖南衡阳421001 [2]南华大学生物化学与分子生物学教研室 [3]南华大学药学系

出　　处：《中南医学科学杂志》2012年第1期17-19,46,共4页Medical Science Journal of Central South China

基　　金：国家自然科学基金(81000328)资助

摘　　要：目的探索槟榔碱对高果糖诱导Wistar大鼠胰岛素抵抗的改善作用。方法采用高糖喂养Wistar大鼠建立胰岛素抵抗大鼠模型。实验大鼠随机分成4组:普通饲料对照组(Control)、高果糖模型组(HF)、普通饲料对照+5 mg/kg槟榔碱组(Con+Are)、高果糖+5 mg/kg槟榔碱组(HF+Are)。Western blot检测GLUT-4和p-PI3K的表达。结果与对照组相比高果糖模型组大鼠骨骼肌葡萄糖转运载体4(GLUT4)和磷酸化磷脂酰肌醇3-激酶(p-PI3K)的表达是明显降低的,而5 mg/kg槟榔碱能明显上调高果糖模型组GLUT-4和p-PI3K的表达,但对对照组无显著影响。结论 5 mg/kg槟榔碱能上调高果糖诱导胰岛素抵抗大鼠骨骼肌细胞GLUT-4和p-PI3K的表达。Objective To investigate the effects of arecoline on high fructose diet inducing insulin resistance in Wistar rats.Methods Insulin resistance（IR） animal model was established by feeding with high fructose diet.The IR model animals were randomly divided into four groups,including control（normal diet,Con）,model（high fructose diet,HF）,normal diet plus 5 mg/ml arecoline（Con＋Are）,and high fructose diet plus 5 mg/ml arecoline（HF＋Are）.Expression of GLUT-4 and p-PI3K in skeletal muscle was measured by western blot.Results Expression of GLUT-4 and p-PI3K in high fructose-fed Wistar rats significantly decreased in comparison with control group,whereas their expression remarkably increased in IR rats treated with 5 mg/ml arecoline,without influence on control group.Conclusions 5 mg/kg arecoline could improve the expression of GLUT-4 and p-PI3K in skeletal muscle of Wistar rats with insulin resistance.

关 键 词：胰岛素抵抗 槟榔碱 GLUT-4 p-PI3K 

分 类 号：R336[医药卫生—人体生理学]