ghrelin抑制ox-LDL诱导的人脐静脉内皮细胞粘附分子表达  

The Inhibitory Effect of Ghrelin on ox-LDL-mediated Adhesion Molecule Expression in hUVECs

作　　者：蒋艳[1] 姜孝新[2] 雷小勇[3] 

机构地区：[1]南华大学附属第一医院药剂科,湖南衡阳421001 [2]南华大学附属第一医院检验科 [3]南华大学药物药理研究所

出　　处：《中南医学科学杂志》2012年第2期144-146,155,共4页Medical Science Journal of Central South China

基　　金：湖南省教育厅课题(11C1110)

摘　　要：目的探讨不同剂量ghrelin预处理对氧化型低密度脂蛋白(ox-LDL)诱导的人脐静脉内皮细胞粘附分子表达的作用。方法人脐静脉内皮细胞经传代培养后随机分配为对照组、ox-LDL组及1 ng/mL、10 ng/mL与100 ng/mL ghreli预处理组,采用逆转录—聚合酶链反应(RT-PCR)检测各组血管内皮细胞粘附分子-1(VCAM-l)与细胞间粘附分子-1(ICAM-1)的RNA水平变化。结果与空白对照组相比:ox-LDL组内皮细胞VCAM-1与ICAM-1分子的表达水平明显升高(P<0.05)。与ox-LDL组相比,1 ng/mL、10 ng/mL、100 ng/mL ghrelin预处理可使内皮细胞VCAM-1 mRNA含量依次降低33%、52%、63%,内皮细胞ICAM-1 mRNA分子表达水平依次降低18%、46%、57%。结论 ghrelin对ox-LDL诱导的人脐静脉内皮细胞中粘附分子VCAM-1及ICAM-1的表达有明显的抑制作用。Objective To investigate the inhibitory effect of different concentration of ghrelin on ox-LDL-mediated adhesion molecule expression in human umbilical vein endothelial cells（hUVECs）.Methods After subculture,hUVECs were randomly divided into control group,ox-LDL group,ghreli-pretreated group of 1 ng/mL,10 ng/mL and 100ng/mL.RT-PCR were used to detect the RNA changes of vasculareen adhesionmolecule-1（VCAM-l） and inter-cellular adhesion molecule-1（ICAM-1）.Results Compared with control group,both VCAM-1 and ICAM-1 expressions were obviously increased（P0.05）.While compared with ox-LDL group,ghrelin-pretreated groups showed a decrease in mRNA level of VCAM-1,which were 33%,52% and 63% respectively by different concentrations of 1,10 and 100 ng/mL ghrelin.The mRNA level of ICAM-1 in ghrelin-pretreated groups were also inhibited,with a decrease of 18%,46% and 57% respectively by different concentrations of 1,10 and 100 ng/mL ghrelin.Conclusion Ghrelin could repress the expression of VCAM-1and ICAM-1 induced by ox-LDL in human umbilical vein endothelial cells.

关 键 词：动脉粥样硬化 GHRELIN 血管内皮细胞粘附分子-1 细胞间粘附分子-1 

分 类 号：R541.4[医药卫生—心血管疾病]