甘草酸的选择性水解及其与唾液酸形成分子复合物的初步研究  

Study of selective hydrolysis of glycyrrhizin and the molecular complex of glycyrrhizin and sialic acid

作　　者：陈桂玲[1] 赵全义[1] 段瑞哲[1] 

机构地区：[1]兰州大学药学院,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2012年第1期29-34,共6页Journal of Lanzhou University（Medical Sciences）

基　　金：甘肃省自然科学基金(3ZS061-A25-091);兰州大学交叉学科青年创新基金(LZU200527)

摘　　要：目的探讨甘草酸的选择性水解以及甘草酸的药理作用机理。方法从禽类动物肝脏中提取β-葡萄糖醛酸苷酶对甘草酸进行催化水解,用差热分析及光谱法研究甘草酸与唾液酸之间形成分子复合物的特征。结果甘草酸可高效选择水解为单葡萄糖醛酸甘草次酸,同时甘草酸与唾液酸可在溶液中形成分子复合物。结论从禽类动物肝脏中提取β-葡萄糖醛酶水解甘草酸是制备单葡萄糖醛酸甘草次酸的一种切实可行的方法,分子复合物的形成为研究甘草酸的药理作用提供了依据。Objective To investigate the selective hydrolysis of glycyrrhizin and its pharmacologic mechanism.Methodsβ-glucuronidase was extracted from poultry to catalyze the hydrolysis of glycyrrhizin,differential thermal analysis and spectrum method were applied to investigate the characteristics of the complex of glycyrrhizin and sialic acid.Results Glycyrrhizin could be efficiently hydrolyzed to mono-β-glucuronide glycyrrhizin acid and at the same time the molecule complex of glycyrrhizin and sialic acid could be formed in the solution.Conclusion It is feasible to product mono-β-glucuronide glycyrrhizin withβ-glucuronidase extracted from poultry and the formation of molecular complex provides basis for further study on pharmacologic mechanism of glycyrrhizin.

关 键 词：甘草酸 Β-葡萄糖醛酸苷酶 唾液酸 分子复合物 

分 类 号：R966[医药卫生—药理学]