尾叶桉杂种子代DNA提取和SSR引物筛选  

DNA extracting and SSR primer screening of Eucalyptus hybrids

作　　者：李光友[1] 徐建民[1] 吴世军[1] 杜志鹄[1] 韩超[1] 王伟[1] 陆钊华[1] 李宝琦[1] 

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520

出　　处：《中南林业科技大学学报》2012年第2期81-85,共5页Journal of Central South University of Forestry & Technology

基　　金：十二五"高产抗逆桉树"新品种的选育研究(2012BAD01B04-1);广东省林业科技创新项目(2009KJCX004-02);广东省林业标准化(2009-BS01);国家科技成果推广项目"桉树速生丰产地力维持与可持续经营技术推广示范";国家公益行业专项(201104003)

摘　　要：以SSR分子标记法分析尾叶桉(Eucalyptus urophylla)及其杂种间关系,采用改良CTAB法提取总DNA,同时优化提取方法和PCR扩增条件。从文献中选取尾叶桉100对SSR引物中筛选出多态性高、稳定性好的17对引物作为实验尾叶桉杂种材料的SSR分析引物,为进一步对其进行遗传多样性和有效基因资源利用研究奠定基础和提供依据。The total DNA of Eucalyptus urophylla and the hybrids were analyzed using SSR markers. The total DNA was extracted by means of improved CTAB isolation method, the extraction methods and PCR amplification conditions were optimized. 17 primers were screened from 100 primers which were from a published-article existing in Eucalyptus urophylla, which had high polymorphism and stability. The results provide references for genetic diversity research of Eucalyptus urophylla families and single tree using SSR marker.

关 键 词：尾叶桉家系 DNA提取 SSR引物筛选 

分 类 号：S792.39[农业科学—林木遗传育种]