CTGF和TGF-β_1的表达与宫腔粘连形成的相关性  

Correlation between the expression of CTGF and TGF-β_1 and the formation of intrauterine adhesion

作　　者：刘琳琳[1] 刘玉环[1] 蒋东桥[2] 夏恩兰[1] 赵玉婷[1] 宋冬梅[1] 郑杰[1] 彭雪冰[1] 

机构地区：[1]首都医科大学附属复兴医院,北京100038 [2]首都医科大学基础医学院

出　　处：《山东医药》2012年第12期17-19,共3页Shandong Medical Journal

基　　金：首都医科大学基础-临床科研合作基金资助项目(2009JL1008)

摘　　要：目的探讨宫腔粘连(IUA)的发生与结缔组织生长因子(CTGF)和转化生长因子-β1(TGF-β1)的关系,为临床预测和预防术后宫腔再粘连提供理论依据。方法以我院行宫腔镜IUA分离术的中重度IUA患者59例为实验组,正常子宫内膜48例为对照组。采用免疫组化法检测CTGF和TGF-β1在IUA患者的子宫内膜、粘连组织及正常子宫内膜组织中的表达。术后1、3个月进行宫腔镜检查,随访IUA患者的月经改善和宫腔情况。结果 CT-GF和TGF-β1在正常子宫内膜、IUA的子宫内膜和粘连组织中均有表达,阳性表达率分别为75.00%和78.80%、80.60%和82.20%、25.93%和74.07%,CTGF和TGF-β1的表达在分泌期子宫内膜高于增殖期子宫内膜;中重度IUA患者的子宫内膜中CTGF和TGF-β1的平均阳性积分高于正常子宫内膜(P<0.01);CTGF与TGF-β1在IUA子宫内膜中的表达呈正相关(r=0.452、P<0.05)。结论 CTGF与TGF-β1参与了IUA的发生发展,且CTGF与TGF-β1的表达有相关性。Objective To explore the relationship between the expression of connective tissue growth factor （CTGF） and transforming growth factor-beta 1 （TGF-β1 ） and the formation of intrauterine adhesion （IUA）. Methods Fifty-nine women with moderate or severe IUA as the study group and forty-eight women with normal endometrium as the control group, all cases underwent hesteroscopic operation in Fuxing hospital. The expression of CTGF and TGF-β1 in the tissues of IUA and the normal endometrium were measured by immunohistochemistry method. The improvement of menstruation and recurrent ad hesion were followed up. Results Positive expression of CTGF and TGF-β1 were found in the normal endometrium, the en- dometrium of IUA patients and the adhesive tissue with rates of 75.00% and 78.80%, 80.60% and 82.20%, 25.93% and 74.07%, respectively. The positive expression of CTGF and TGF-131 in the secretory endometrium were higher than those in the proliferative endometrium. The average positive integral of CTGF and TGF-β1 in the endometrium of IUA patients were higher than those in the normal endometrium with significant difference （ P 〈 0.01 ）. The analysis of immunohistochemistry results in the endometrium of IUA patients showed positive correlation between the expression of CTGF and TGF-β1 （r = 0. 452, P 〈0.05）. Conclusions The expression of CTGF and TGF-β1are correlated with the occurrence and development of IUA. The expression of CTGF was correlated with that of TGF-β1in the endometrium of IUA.

关 键 词：宫腔粘连 转化生长因子-Β1 结缔组织生长因子 免疫组织化学 

分 类 号：R711.74[医药卫生—妇产科学]